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商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:60kDa(CDK5),50kDa(p25)
Genbank#:NM_004935,NM_003885
Tag(s):N-terminalGST-tag
a.a:CDK51–292(end),p2599–307(end)
Background:
CDK5isamemberoftheCyclin-DependentKinasefamilythatismostabundantinthemammalianbrain.ActiveformofCDK5,whichhasalsobeencalledneuronalcdc2-likekinase,isaheterodimerofCDK5anda25kDaproteinwhichisderivedproteolyticallyfroma35kDabrainandneuron-specificproteinandisessentialforthekinaseactivityofCDK5(1).CDK5hasemergedasacrucialregulatorofneuronalmigrationinthedevelopingcentralnervoussystem.CDK5phosphorylatesadiverselistofsubstrates,implicatingitintheregulationofarangeofcellularprocesses-fromadhesionandmotility,tosynapticplasticityanddrugaddiction(2).
Description:
ComplexofrecombinantfulllengthhumanCDK5(GenBankAccessionNo.NM_004935),a.a.1-292(end)withN-terminalGST-tagandp25(GenBankAccessionNo.NM_003885),a.a.99-307(end)withN-terminalGST-tag.Co-expressedinaBaculovirus-infectedSf9cellexpressionsystem.CDK5MW=60kDa,p25MW=50kDa.
UniProt
Q00535(CDK5),Q15078(p35)
Synonym(s):
Cyclin-DependentKinase-5,CDK5:PSSALRE
p25:CDK5R1;CDK5P35,CDK5R,NCK5A,p23,p35,p35nck5a
p25:CDK5R1;CDK5P35,CDK5R,NCK5A,p23,p35,p35nck5a
SpecificActivity:
312pmol/min/μg
AssayConditions:
AssaywasdoneinaKinasebufferusingHistoneH1(BPScat.#52043)asasubstratewith20μMATPat30°Cfor30min.TheamountofATPtransferredwascalculatedusingKinase-Glo®reagent(Promega).
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,4mMglutathione,0.04%Tween-20,and20%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Tang,D.etal:Cyclin-dependentkinase5(Cdk5)andneuron-specificCdk5activators.ProgCellCycleRes.1996;2:205-16.
2.Dhavan,R.etal:AdecadeofCDK5.NatRevMolCellBiol.2001Oct;2(10):749-59.
2.Dhavan,R.etal:AdecadeofCDK5.NatRevMolCellBiol.2001Oct;2(10):749-59.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
