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商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:68kDa
Genbank#:NM_153498
Tag(s):N-terminalGST-tag
a.a:fulllength
Background:
CAMK1δorCa2+/calmodulin-dependentkinaseI-likekinase(CKLiK)isactivatedbyCa(2+)andcalmodulinandisdetectedinCD34+-derivedneutrophilsandeosinophils,aswellasinmatureperipheralbloodgranulocytes(1).CAMK1δexhibitsCa(2+)/CaM-dependentactivitythatisenhancedinvitrobyphosphorylationofitsThr180byCaM-Kkinase(CaM-KK)alpha,consistentwithdetectionofCAMK1D-activatingactivityinHeLacells(2).
Description:
RecombinanthumanCAMK1δ,fulllength,withN-terminalGST-tag,expressedinSf9insectcellsviaabaculovirusexpressionsystem.
Synonym(s):
RP11-462F15.1,CKLiK,CaM-K1,CaMKID
Purity:
≥75%
Formulation:
50mMTris-HCl,pH7.5,150mMNaCl,0.25mMDTT,0.1mMEGTA,0.1mMEDTA,0.1mMPMSF,25%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Verploegen,S.etal:CharacterizationoftheroleofCaMKI-likekinase(CKLiK)inhumangranulocytefunction.Blood106(3),1076-1083(2005).
2.Ishikawa,Y.etal:IdentificationandcharacterizationofnovelcomponentsofaCa2+/calmodulin-dependentproteinkinasecascadeinHeLacells.FEBSLett.550(1-3),57-63(2003).
2.Ishikawa,Y.etal:IdentificationandcharacterizationofnovelcomponentsofaCa2+/calmodulin-dependentproteinkinasecascadeinHeLacells.FEBSLett.550(1-3),57-63(2003).
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
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