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商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:67kDa
Genbank#:NM_032019
Tag(s):N-terminalFLAG-tag
a.a:2-631
Description:
HumanHDAC10(a.a.2-631)(GenBankAccessionNo.NM_032019)withN-terminalFLAG-tag,MW=67kDa,expressedinSf9cellsviaabaculovirusexpressionsystem.
UniProt
Q969S8
Synonym(s):
histonedeacetylase10,HDAC10
SpecificActivity:
≥3.6pmol/min/μg.
AssayConditions:
25mMTris/Cl,pH8.0,137mMNaCl,2.7mMKCl,1mMMgCl2,and0.1mg/mlBSA,20μMBPSHDACsubstrate3(Catalog#50037),and40ng/μlHDAC10.Incubationcondition:30min.at37ºC.
Formulation:
45mMTris-HCl,pH8.0,124mMNaCl,2.4mMKCl,0.05%Tween-20and10%glycerol.
Format:
Aqueousbuffersolution
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.ChakrabortyS.(2006)J.Biol.Chem.281(46):35070-80.
2.Wolfgang,F.(2001)J.Biol.Chem.276(38):35826-35835.
2.Wolfgang,F.(2001)J.Biol.Chem.276(38):35826-35835.
ApplicationReference(s):
1.Suppressionofmonosodiumuratecrystal-inducedcytokineproductionbybutyrateismediatedbytheinhibitionofclassIhistonedeacetylases(2015)
2.Theeffectofvariouszincbindinggroupsoninhibitionofhistonedeacetylases1-11(2014)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Deacetylase
ProductType:
RecombinantProtein
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