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商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:77kDa
Genbank#:NM_007365
Tag(s):C-terminalFLAG-tag
a.a:1–665(end)
Description:
HumanPADI2,alsoknownasPeptidylargininedeiminase,typeII,(GenBankAccessionNo.NM_007365),1-665(end),withC-terminalFLAG-tag,MW=77kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
UniProt
Q9Y2J8
Synonym(s):
PADI-2,Peptidylargininedeiminase,typeII,Pad
SpecificActivity:
≥19pmole/min/µg.
AssayConditions:
Enzymereactionwasperformedinabuffer(50mMTris,pH7.4,50mMNaCl,10mMCaCl2,and2mMDTT)containingPADIand10mMBAEEasasubstratefor30minat37°C.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,80ng/µlFLAGpeptide,3mMDTT,and20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.DongS,etal.(2005.)J.Invest.Dermatol.124(5):1026-33.
2.WantanabeY,etal.(2009).JHumGenet.54(7):430–2.
2.WantanabeY,etal.(2009).JHumGenet.54(7):430–2.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
ArginineDeiminase
ProductType:
RecombinantProtein
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