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商品描述
Species:Human
HostSpecies:Sf9cells
MW:87kDa
Genbank#:NM_024420
Tag(s):C-terminalFLAG-His-tags
a.a:1-749(end)
Description:
HumanPLA2G4A,GenBankAccessionNo.NM_024420,a.a.1-749(end),withC-terminalFLAG-His-tag,MW=87kDa,expressedinabaculovirusexpressionsystem.
UniProt
P47712
Synonym(s):
PhospholipaseA2G4
SpecificActivity:
≥57pmol/min/µg.
AssayConditions:
PLAreactionwasperformedusingthe1-Hexadecanoyl-2-(1-prenedecanoyl)-sn-Glycero-3-phosphocholinesubstrate.Enzymereactionisconductedin100µlvolumecontaining50mMTrispH8.0,500mMNaCl,10mMCaCl2,0.5mg/mlBSA,10µMsubstrateand200ngPLA2G4A.Reactionprogresswasmonitoredatex345/em395for10minutescontinuouslyatroomtemperature.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,80µg/mlFLAGpeptide,and20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Reference(s):
1.ChalimoniukM.PostepyBiochem.2012;58(2):204-8.
2.NadalinS,GiacomettiJ,etal.,ProstaglandinsLeukotEssentFattyAcids.2013;88(2):185-90.
2.NadalinS,GiacomettiJ,etal.,ProstaglandinsLeukotEssentFattyAcids.2013;88(2):185-90.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Phospholipases
ProductType:
RecombinantProtein
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