品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Species:Human
HostSpecies:Sf9insectcells
MW:92kDa
Genbank#:NM_001048201
Tag(s):N-terminalHis-FLAG-tags
a.a:2–793
Background:
MultidomainproteinthatactsasakeyepigeneticregulatorbybridgingDNAmethylationandchromatinmodification.SpecificallyrecognizesandbindshemimethylatedDNAatreplicationforksviaitsYDGdomainandrecruitsDNMT1methyltransferasetoensurefaithfulpropagationoftheDNAmethylationpatternsthroughDNAreplication.InadditiontoitsroleinmaintenanceofDNAmethylation,italsoplaysakeyroleinchromatinmodification.TheproteinbindstospecificDNAsequences,andrecruitsahistonedeacetylasetoregulategeneexpression.HasE3ubiquitin-proteinligaseactivitybymediatingtheubiquitinationoftargetproteinssuchashistoneH3andPML.MaybeinvolvedinDNArepair.ItsexpressionpeaksatlateG1phaseandcontinuesduringG2andMphasesofthecellcycle.ItplaysamajorroleintheG1/StransitionbyregulatingtopoisomeraseIIalphaandretinoblastomageneexpression,andfunctionsinthep53-dependentDNAdamagecheckpoint.AlsolocalizestoeuchromaticregionswhereitnegativelyregulatestranscriptionpossIBLybyimpactingDNAmethylationandhistonemodifications.
Description:
Humanubiquitin-likewithPHD,Tudor andringfingerdomains1(UHRF1),
GenBankAccessionNo.NM_001048201,a.a.2–793(end)with
N-terminalHis-Flagtag,MW=92kDa, expressedinaBaculovirusinfectedSf9
cellexpressionsystem.
UniProt
Q96T88
Synonym(s):
UHRF1,Ubiquitin-LikeWithPHDAndRingFingerDomains,RINGFingerProtein106,RNF106,Np95,ICBP90
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,0.05%Tween-20,100μg/mlFlagpeptide,and20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
≥ 6monthsat-80°C
Application(s):
Usefulforproteinbindingassays,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Kofunato,Y., etal.,OncolRep.2012Dec; 28(6):1997-2002.
2.Jazirehi,A.R., etal.,Epigenomics.2012Jun;4(3):251-252.
3.Jenkins,Y., etal.,MolBiolCell.2005Dec;16(12):5621-9.
ApplicationReference(s):
Poly(ADP-ribose)polymerase1(PARP1)associateswithE3ubiquitin-proteinligaseUHRF1andmodulatesUHRF1BIOLOGicalfunctions(2014)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Methyl-lysineReader,UbiquitinE3ligase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
