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商品描述
Species:Human
HostSpecies:E.coli
MW:47.5kDa
Genbank#:NM_001048201
Tag(s):N-terminalGST-tag
a.a:108–286
Background:
MultidomainproteinthatactsasakeyepigeneticregulatorbybridgingDNAmethylationandchromatinmodification.SpecificallyrecognizesandbindshemimethylatedDNAatreplicationforksviaitsYDGdomainandrecruitsDNMT1methyltransferasetoensurefaithfulpropagationoftheDNAmethylationpatternsthroughDNAreplication.InadditiontoitsroleinmaintenanceofDNAmethylation,italsoplaysakeyroleinchromatinmodification.TheproteinbindstospecificDNAsequences,andrecruitsahistonedeacetylasetoregulategeneexpression.HasE3ubiquitin-proteinligaseactivitybymediatingtheubiquitinationoftargetproteinssuchashistoneH3andPML.MaybeinvolvedinDNArepair.ItsexpressionpeaksatlateG1phaseandcontinuesduringG2andMphasesofthecellcycle.ItplaysamajorroleintheG1/StransitionbyregulatingtopoisomeraseIIalphaandretinoblastomageneexpression,andfunctionsinthep53-dependentDNAdamagecheckpoint.AlsolocalizestoeuchromaticregionswhereitnegativelyregulatestranscriptionpossIBLybyimpactingDNAmethylationandhistonemodifications.
Description:
TandemTudorDomainregion(a.a.108-286)ofhumanubiquitin-likewithPHDandringfingerdomains1(UHRF1),GenBankAccession#NM_001048201,withN-terminalGST-tag,MW=47.5kDa,expressedinanE.colicellexpressionsystem.
UniProt
Q96T88
Synonym(s):
UHRF1,Ubiquitin-LikeWithPHDAndRingFingerDomains,RINGFingerProtein106,RNF106,Np95,ICBP90
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,10mMβ-mercaptoethanol,20mMglutathione,and20%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
≥ 6monthsat-80°C
Application(s):
Usefulforproteinbindingassays,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Kofunato,Y.,etal.,OncolRep.2012Dec;28(6):1997-2002.
2.Jazirehi,A.R.,etal.,Epigenomics.2012Jun;4(3):251-252.
ApplicationReference(s):
Poly(ADP-ribose)polymerase1(PARP1)associateswithE3ubiquitin-proteinligaseUHRF1andmodulatesUHRF1BIOLOGicalfunctions(2014)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Methyl-lysineReader
ProductType:
RecombinantProtein
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