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蚂蚁淘在线 / 品牌中心 / BPS Bioscience / Bpsbioscience/SIRT5 (Sirtuin5) Fluorogenic Assay Kit/50085/96 reactions
商品描述
Description:
TheFluorogenicSIRT5AssayKitisacompleteassaysystemdesignedtomeasureSIRT5activityforscreeningandprofilingapplications.TheFluorogenicSIRT5AssayKitisbasedonauniquefluorogenicsubstrateanddevelopercombination.ThisassaymethodeliminatesdealingwiththerADIoactivity,extraction,andchromatographyaspectsoftraditionalassays.Usingthiskit,onlythreesimplestepsonamicrotiterplateareneededtoanalyzetheSIRT5activitylevel.First,theSirtuin5fluorometricsubstrate(SIRTsubstrate5),containingasuccinylatedlysinesidechain,isincubatedwithpurifiedSIRT5enzyme.ThedesuccinylationsensitizesthesubstratesosubsequenttreatmentwiththeLysineDeveloperproducesafluorophorethatcanthenbemeasuredusingafluorescencereader.
UniProt
Q9NXA8
Synonym(s):
sirt5,sirtuin5
SuppliedAs:
TheFluorogenicSIRT5AssayKitcomesinaconvenient96-wellformat,withallthereagentsnecessaryfor100fluorescentSIRT5activitymeasurements.
Contraindications:
DMSO>1%,strongacidsorbases,ionicdetergents,highsalt
Format:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
1.J.Duetal.(2011)Science.334:806.
2.A.Madsenetal.(2012)J.Med.Chem55:5582.
3.F.Fisheretal.(2012)PlosOne.7:1.
2.A.Madsenetal.(2012)J.Med.Chem55:5582.
3.F.Fisheretal.(2012)PlosOne.7:1.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Deacetylase
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
