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蚂蚁淘在线 / 品牌中心 / BPS Bioscience / Bpsbioscience/SIRT3 (Sirtuin3) Fluorogenic Assay Kit/50088/32 reactions
商品描述
Description:
TheFluorogenicSIRT3AssayKitisacompleteassaysystemdesignedtomeasureSirtuin3(SIRT3)activityforscreeningandprofilingapplications.TheFluorogenicSIRT3AssayKitisbasedonauniquefluorogenicsubstrateanddevelopercombination.ThisassaymethodeliminatesdealingwiththerADIoactivity,extraction,andchromatographyaspectsoftraditionalassays.Usingthiskit,onlytwosimplestepsonamicrotiterplateareneededtoanalyzetheSIRT3activitylevel.First,theHDACfluorometricsubstrate(HDACsubstrate1),containinganacetylatedlysinesidechain,isincubatedwithpurifiedSIRT3enzyme.ThedeacetylationsensitizesthesubstratesosubsequenttreatmentwiththeLysineDeveloperproducesafluorophorethatcanthenbemeasuredusingafluorescencereader.
UniProt
Q9NTG7
Synonym(s):
sirt3,sirtuin3
SuppliedAs:
Kitcomesinaconvenient96-wellformat,withallthereagentsnecessaryfor32fluorescentSIRT3activitymeasurements.
Contraindications:
DMSO>1%,strongacidsorbases,ionicdetergents,highsalt
Format:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
1.A.Itoetal.(2001)EMBOJ.201331.
2.A.Itoetal.(2002)EMBOJ.216236.
3.N.A.Barlevetal.(2001)Mol.Cell81243.
ApplicationReference(s):
1. RegulationofSIRT3signalrelatedmetabolicreprogrammingingastriccancerbyHelicobacterpylorioncoproteinCagA(2017)
2. Inhibitionofmyocardialreperfusioninjurybyischemicpostconditioningrequiressirtuin3-mediateddeacetylationofcyclophilinD(2015)
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Deacetylase
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
