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苏州工业园区生物纳米园A4#216
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0512-67156496
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商品描述
Species:Human
HostSpecies:Freestyle293cells
MW:~150kDa
Tag(s):none
Clonality:Monoclonal(human)
Isotype:IgG1
Background:
PCSK9isacrucialplayerintheregulationofplasmacholesterolhomeostasis.Itbindstolow-densitylipidreceptorfamilymembers:lowdensitylipoproteinreceptor(LDLR),verylowdensitylipoproteinreceptor(VLDLR),apolipoproteinEreceptor(LRP1/APOER)andapolipoproteinreceptor2(LRP8/APOER2),andpromotestheirdegradation.Actsviaanon-proteolyticmechanismtoenhancethedegradationofthehepaticLDLRthroughaclathrinLDLRAP1/ARH-mediatedpathway.MaypreventtherecyclingofLDLRfromendosomestothecellsurfaceordirectittolysosomesfordegradation.CaninduceubiquitinationofLDLRleADIngtoitssubsequentdegradation.InhibitsintracellulardegradationofAPOBviatheautophagosome/lysosomepathwayinaLDLR-independentmanner.Involvedinthedisposalofnon-acetylatedintermediatesofBACE1intheearlysecretorypathway.InhibitsepithelialNa(+)channel(ENaC)-mediatedNa(+)absorptionbyreducingENaCsurfaceexpressionprimarilybyincreasingitsproteasomaldegradation.RegulatesneuronalapoptosisviamodulationofLRP8/APOER2levelsandrelatedanti-apoptoticsignalingpathways.
Description:
PCSK9NeutralizingAntibody,aneutralizingrecombinanthumanantibody(IgG1)recogniziestheLDLRbindingregionofhumanPCSK9.
Synonym(s):
Anti-PCSK9
AssayConditions:
3.8ng/µlbiotinylatedPCSK9wasincubatedwithvariousconcentrationsofAnti-PCSK9for1hrina50µlreactionmix.MixturewasincubatedonLDLRcoatedplatefor2hr.StreptavidinlabeledHRPwasaddedandincubatedfor1hr.Finally,HRPchemiluminescentsubstrateswereaddedandluminescencewasread.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,and20%glycerol.
Immunogen:
LDLRbindingregionofhumanPCSK9
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
UsefulasaninhibitorofPCSK9-LDLRbindingusingthePCSK9[Biotinylated]-LDLRBindingAssayKit,Cat.#72002.
Reference(s):
1.Banerjee,Y.,etal.,NEnglJMed.2012Jun21,366(25):2425-6.
2.Maxwell,K.N.,Breslow,J.L.,CircRes.2012Jul20,111(3):274-7.
ApplicationReference(s):
1.Cell-based,bioluminescentassayformonitoringtheinteractionbetweenPCSK9andtheLDLreceptor(2017)
2. PCSK9isacriticalregulatoroftheinnateimmuneresponseandsepticshockoutcome(2014)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Cholesterol-Related
ProductType:
Inhibitor
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