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蚂蚁淘在线 / 品牌中心 / BPS Bioscience / Bpsbioscience/BRPF3 Inhibitor Screening Assay Kit/32608/384 reactions
商品描述
Description:
TheBRPF3InhibitorScreeningAssayKitisdesignedtomeasuretheinhibitionofBRPF3bindingtoitssubstrate.ThekeytotheBRPF3InhibitorScreeningAssayKitisthespecificbindingoftheBRPF3bromodomaintoanacetylatedhistonesubstrate.Withthiskit,onlythreesimplestepsonamicrotiterplatearerequired.First,asamplecontainingBRPF3andaninhibitorofchoiceisincubatedwiththebiotinylatedsubstrate.Next,acceptorbeadsareadded,thendonorbeads,followedbyreADIngtheAlpha-counts
Synonym(s):
BRPF3,BromodomainandPHDfingercontaining3
SuppliedAs:
ThekitcomesinaconvenientAlphaLISA®format,withbiotinylatedhistonepeptidesubstrate,assaybuffer,detectionbufferandpurifiedGST-taggedBRPF3bromodomaintoperformatotalof384enzymereactions.
Contraindications:
DMSOabove0.5%.OnlylimitedamountsofDMSOcanbeincluded,asithasbeenshowntodisruptBRD-ligandinteraction.AvoidgreenandbluedyesthatabsorblightintheAlphascreen®signalemissionrange(520-620nm),suchasTrypanBlue.Avoidtheuseofthepotentsingletoxygenquencherssuchassodiumazide(NaN3)ormetalions(Fe2+,Fe3+,Cu2+,Zn2+andNi2+).Thepresenceof>1%RPMI1640culturemediumleadstoasignalreductionduetothepresenceofexcessbiotinandironinthismedium.MEM,whichlacksthesecomponents,doesnotaffectAlphascreen®assays.
Format:
COMPONENTS:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
Atleast12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
Usefulforthestudyofbromodomainbindingassays,screeningbromodomaininhibitorsandselectivityprofiling.
Reference(s):
Filippakopoulos,P.,etal.,Cell2012;149:214.
Notes:
MATERIALSORINSTRUMENTSREQUIREDBUTNOTSUPPLIED:
GlutathioneAlphaLISA®AcceptorBeads,5mg/ml(Perkinelmer#AL109C)
AlphaScreen®Streptavidin-conjugateddonorbeads,5mg/ml(PerkinElmer#6760002)
Optiplate-384(PerkinElmer#6007290)
AlphaScreen®microplatereader
Adjustablemicropipettorandsteriletips
GlutathioneAlphaLISA®AcceptorBeads,5mg/ml(Perkinelmer#AL109C)
AlphaScreen®Streptavidin-conjugateddonorbeads,5mg/ml(PerkinElmer#6760002)
Optiplate-384(PerkinElmer#6007290)
AlphaScreen®microplatereader
Adjustablemicropipettorandsteriletips
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Bromodomain
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
