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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Description:
TheBRDT(BD1+BD2)TR-FRETAssayKitisdesignedtomeasuretheinhibitionofBRDT(BD1+BD2)bindingtoitssubstrateinahomogeneous384reactionformat.ThisFRET-basedassayrequiresnotime-consumingwashingsteps,makingitespeciallysuitableforhighthroughputscreeningapplications.Theassayprocedureisstraightforwardandsimple;asamplecontainingterbium-labeleddonor,dye-labeledacceptor,BRDT,ligand,andaninhibitorisincubatedforsixtyminutes.Then,thefluorescenceintensityismeasuredusingafluorescencereader.
Synonym(s):
Bromodomaintestis-specificprotein,BRDT,BRD6,CT9
SuppliedAs:
KitcomesinaconvenientformatwithpurifiedBRDTprotein,ligands,Tbdonor,dye-labeledacceptor,assaybufferandmicrotiterplatetoperform384reactions.
Format:
COMPONENTS:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
Atleast12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
Filippakopoulos,P.,etal.,Cell2012;149:214.
Notes:
MATERIALSORINSTRUMENTSREQUIREDBUTNOTSUPPLIED:
FluorescentmicroplatereadercapableofmeasuringTimeResolvedFluorescence ResonanceEnergyTransfer(TR-FRET)
Adjustablemicropipettorandsteriletips
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Bromodomain
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
