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苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
HostSpecies:Mouse
Clonality:monoclonal
Isotype:IgG1
Background:
EZH2(UniProt/Swiss–ProtentryQ15910)isahistone–lysinemethyltransferasewhichmethylatesLys–9andLys–27ofhistoneH3,leADIngtotranscriptionalrepression.Itisamemberofthepolycombgroup(PcG)familywhichformmultimericproteincomplexesandareinvolvedinmaintainingthetranscriptionalrepressivestateofgenesoversuccessivecellgenerations.TheEZH2activityisdependentontheassociationwithothercomponentsofthePRC2complex(EED,SUZ12/JJAZ1,RBBP4andRBBP7).EZH2mayplayaroleinthehematopoieticandcentralnervoussystems.Over–expressionofEZH2isobservedduringadvancedstagesofprostatecancerandbreastcancer.
Description:
MonoclonalantibodyraisedinmouseagainstthecentralpartofthehumanEZH2protein(Enhancerofzestehomolog2)
Synonym(s):
ENX–1,KMT6
AssayConditions:
ChIPresultsobtainedwiththemonoclonalantibodydirectedagainstEZH2
ChIPassayswereperformedusing2.5µgofthemonoclonalantibodyagainstEZH2(Cat.#25222)onshearedchromatinfrom5millionE14mouseembryonicstemandfromSUZ12-/-cells,usedasanegativecontrol.IgGwasusedasnegativeIPcontrol.QuantitativePCRwasperformedwithprimersspecificfortheHoxA10geneandfortheTgenewhichencodestheBrachyurytranscriptionfactor.Figure1showstherecovery,expressedasa%ofinput(therelativeamountofimmunoprecipitatedDNAcomparedtoinputDNAafterqPCRanalysis).
Concentration:
50µg/50µl
Formulation:
PBScontaining0.05%azide
SpeciesReactivity:
Human,mouse
Purification:
ProteinGpurified
Immunogen:
recombinantprotein
Format:
Aqueousbuffersolution
Storage/StABIlity:
Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.
Application(s):
ChIP(2.5µg/ChIP)
Notes:
TheoptimalantibodyamountperIPshouldbedeterminedbytheend–user.Werecommendtesting1–5µgperIP
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Antibodies
ProductType:
Antibody
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