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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
HostSpecies:Mouse
Clonality:monoclonal
Background:
Theestrogenreceptoralpha(ERalpha,UniProt/Swiss–ProtentryP03372)belongstothefamilyofnuclearhormonereceptors,whichareligand–activatedtranscriptionfactors.Theyareimportantfortheregulationofgeneexpression,cellularproliferationanddifferentiation,sexualdevelopmentandreproductivefunction.Estrogenreceptorsarealsoinvolvedinpathologicalprocessessuchasbreastcancer,andosteoporosis.ERalphacanregulatetranscriptionbydirectbindingtoestrogenresponseelements(EREs)intheDNAorbyinteractionwithothertranscriptionfactors.ItmayalsoformaheterodimerwithERbeta.
Description:
MonoclonalantibodyraisedinmouseagainsthumanERalpha(estrogenreceptoralpha),usingasyntheticpeptide
Synonym(s):
ESR,ESR1,ESRA,NR3A14
AssayConditions:
ChIP-seqresultsobtainedwiththemonoclonalantibodydirectedagainstERalpha
ChIPwasperformedwiththemonoclonalantibodyagainstERalpha(Cat.#25221)onshearedchromatinfromMCF7cellstreatedfor1hourwithestrADIol.TheimmunoprecipitatedDNAwasanalyzedwithanIlluminaGenomeAnalyzer.Librarypreparation,clustergenerationandsequencingwereperformedaccordingtothemanufacturer"sinstructions.The36bptagswerealignedtothehumangenomeusingtheELANDalgorithm.Figure1showstheobtainedpeaksneartheTFF1geneonchromosome21(figure1A),theGREB1andHAAOgenesonchromosome2(figure1BandC),andtheZNF185geneontheX-chromosome(figure1D).
Concentration:
100µl
Formulation:
Ascitesfluidfrommousecontaining0.05%azide
SpeciesReactivity:
Human
CrossReactivity:
Doesnotreactwithchicken;otherspeciesnottested
Purification:
Ascitesfluid
Immunogen:
syntheticpeptide
Format:
aqueoussolution
Storage/StABIlity:
Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.
Application(s):
ChIP/ChIP–seq(2.5µl)
ELISA(1:500–1:5000)
WB(1:500–1:5000)
GelSupershift(1:10–1:20)
Immunochemistry(1:500–1:5000)
IP(1:200–1:5000)
ELISA(1:500–1:5000)
WB(1:500–1:5000)
GelSupershift(1:10–1:20)
Immunochemistry(1:500–1:5000)
IP(1:200–1:5000)
Notes:
TheoptimalantibodyamountperIPshouldbedeterminedbytheend–user.Werecommendtesting1–5µgperIP
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Antibodies
ProductType:
Antibody
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