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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
HostSpecies:Rabbit
Clonality:polyclonal
Background:
3–methylcytosinearisesbyerroneousmethylationofDNAthroughspontaneousexposuretoendogenousS–adenosylmethionine(SAM).SincetheN3–positionincytosineisprotectedindoublestrandedDNA,thisphenomenonmainlyoccursinsinglestrandedDNA.Theresulting3–mCistoxicandmutagenicandincreasedlevelsof3–mCseemtoimpaircellproliferation.Therefore,ithastoberepairedbythecell.Thisisestablishedbyoxidativedemethylation,whichiscatalyzedbytheAlkBprotein.
Description:
Polyclonalantibodyraisedinrabbitagainst3–methylcytosine(3–mC)conjugatedtoBSA.
AssayConditions:
Dotblotanalysisusingtheantibodydirectedagainst3-mC
Todemonstratethespecificityoftheantibodyagainst3-mC(cat.No.25200),aDotBlotanalysiswasperformedusingsyntheticoligonucleotidescontainingdifferentmodifiedbases.150and50pmoloftherespectiveoligo’swerespottedonthemembrane.Theantibodywasdiluted1:400inPBS-Tcontaining10%skimmedmilkand1%BSA.Figure1showsahighspecificityoftheantibodyfortheoligonucleotidewiththe3-methylcytosinemodification.
Concentration:
50µg/28µl
Formulation:
PBScontaining0.05%azideand0.05%ProClin300.
SpeciesReactivity:
Human,mouse,broadrange
CrossReactivity:
Doesnotcross–reactwith5–methylcytosine,5–hydroxymethylcytosine,or5–carboxylcytosine.
Purification:
ProteinGpurified
Immunogen:
syntheticpeptide
Format:
Aqueousbuffersolution
Storage/StABIlity:
Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.
Application(s):
DB(1:400)
Notes:
Theoptimalantibodyconcentrationshouldbedeterminedbytheend–user.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Antibodies
ProductType:
Antibody
Datashownislot-specific.Contactusforspecificinformationonotherlots.
