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商品描述
Species:Human
HostSpecies:E.coli
MW:28kDa
Genbank#:Z23115
Tag(s):C-terminalHis-tag
a.a:1–212
Background:
BCL-2familymembersformhetero-orhomodimersandactasanti-orpro-apoptoticregulatorsthatareinvolvedinawidevarietyofcellularactivities.Theproteinsencodedbythisgenearelocatedattheoutermitochondrialmembrane,andhavebeenshowntoregulateoutermitochondrialmembranechannel(VDAC)opening.VDACregulatesmitochondrialmembranepotential,andthuscontrolstheproductionofreactiveoxygenspeciesandreleaseofcytochromeCbymitochondria,bothofwhicharethepotentinducersofcellapoptosis.Twoalternativelysplicedtranscriptvariants,whichencodedistinctisoforms,havebeenreported.Thelongerisoform(Bcl-xL)actsasanapoptoticinhibitorandtheshorterform(Bcl-xS)actsasanapoptoticactivator.
Description:
HumanBCL-XL(GenBankAccessionNo.Z23115),a.a.1-212withC-terminalHis-tag,MW=28kDa,expressedinanE.coliexpressionsystem.
UniProt
Q07817
Synonym(s):
Bcl-Xlong,BcelllymphomaXL,bclxl
AssayConditions:
FollowingtheprotocolforBPSCat.#50223.Briefly,BCL-XLwascombinedwithBCL-XLpeptideligandinTR-FRETassaybufferalongwithanti-HisTbdonoranddye-labeledacceptor.VaryingconcentrationsofABT199wereaddedandthereactionwasincubatedatroomtemperaturefor2hoursfollowedbyfluorescentintensitymeasurements.
Formulation:
45mMTris-HCl,pH8.0,124mMNaCl,2.4mMKCl,10%glycerol,225mMimidazoleand3mMDTT.
Format:
Aqueousbuffersolution
Application(s):
Usefulforthestudyofapoptosis,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.MengY.etal.,MolCancerTher.2008Jul;7(7):2192-202.
2.AsakuraT.etal.,MolIntJOncol.2008Aug;33(2):389-95.
2.AsakuraT.etal.,MolIntJOncol.2008Aug;33(2):389-95.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Apoptosis
ProductType:
RecombinantProtein
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