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苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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商品描述
Background:
GCN5(alsoknownasKAT2A)isinvolvedinvariouscellularevents,anditsdysfunctionislinkedtoanumberofhumandiseasesincludingcancersanddiabetes.GCN5isahistoneacetyltransferasethatfunctionsprimarilyasatranscriptionalactivator.ItalsofunctionsasarepressorofNF-kappa-BbypromotingubiquitinationoftheNF-kappaBsubunitRelAinaHAT-independentmanner.
Description:
TheGCN5ChemiluminescentAssayKitisanenzyme-linkedimmunosorbentassay(ELISA)designedtoscreenforinhibitorsofGCN5.TheGCN5ChemiluminescentAssayKitisbasedontheGCN5enzymetransferringanacetylgroupfromanacetyldonor(acetylCoA)toahistonesubstrate.Theacetylatedhistoneisrecognizedbyahighlyspecificprimaryantibody,followedbyanHRP-labeledsecondaryantibody.ThechemiluminescenceproducedbyHRPcanbemeasuredusingachemiluminescencereader.
Synonym(s):
GCN5,KAT2A,GCNL2,PCAF-β,PCAF-B
SuppliedAs:
Kitcomeswitha96-wellplateprecoatedwithNeutravidinandHistonepeptide,andallthereagentsnecessaryfor96chemiluminescentGCN5activitymeasurements.Inaddition,thekitincludespurifiedGCN5foruseasapositivecontrol.
Contraindications:
DMSO>1%,strongacidsorbases,ionicdetergents,highsalt.
Format:
COMPONENTS:
Storage/StABIlity:
Stableatleast6monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymeactivityandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
1.Chen,L.,etal.(2013).J.Biol.Chem.17(20):14510-21.
2.Dominy,J.E.Jr.,etal.(2010).Biochim.Biophys.Acta.1804(8):1676-83.
2.Dominy,J.E.Jr.,etal.(2010).Biochim.Biophys.Acta.1804(8):1676-83.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Acetyltransferase
ProductType:
AssayKit
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