08:0 DGPP

dioctanoylglycerol pyrophosphate (ammonium salt)

Diacylglycerol pyrophosphate (DGPP), involved in a novel signaling pathway, has been found in plants, Saccharomyces cerevisiae, and Escherichia coli and macrophage-activating factor, and suggests a potential role for this compound in triggering homeostatic cellular responses.

Biological Applications:

Short chain DGPP (DiC8)

DiC8 DGPP is added to a test tube as a chloroform solution and the solvent is removed. Add water or buffer to the dry lipid residue followed by sonication. The solution is added to cells directly. The short chain DGPP is taken up by mammalian cells and by yeast.

Long chain DGPP (DiC18:1)

DiC18:1 DGPP is added to a test tube as a chloroform solution and the solvent is removed. Add 0.5% Tergitol (detergent) solution and add this to cells.

Notes:

Biological Application — "I am not sure how well the long chain DGPP is taken up in yeast cells, that is why we synthesized the DiC8 compound. We only use the long chain compound as a standard for TLC, HPLC and for DGPP phosphatase assays".

Storage Conditions — "Store concentrated, ~25mg/ml in chloroform. Concentrations below 10mg/ml are not as stable".

Personal communication, George M. Carman, Sept. 1999

供应Avanti脂质体挤出器，进口实验室仪器设备，美国Avanti Polar Lipids公司提供的MiniExtruder脂质体制备装置。Avanti Polar Lipids的mini-extruder允许研究人员和科学家准备大单层脂质体，挤在一个高效，快速的方式。持有人/加热块允许挤压的单层脂质体在高温下，这是至关重要的成功生产囊泡从磷脂相变温度高于室温。 配置： 迷你挤压机 1个 气密注射器 2个 加热模块 1个（610000标配，610023选配） 聚碳酸酯膜 100片 过滤膜支架 100个