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蚂蚁淘在线 / 品牌中心 / Stemcell / Stemcell/EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit/RoboSep/18063RF
商品描述
Overview
This kit isolates human CD4+CD127lowCD25+ regulatory T cells (Tregs) from fresh peripheral blood mononuclear cells (PBMCs) or leukapheresis samples. First, CD25+ cells are isolated by column-free immunomagnetic positive selection using EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD25+ cells, and unwanted non-Tregs are targeted for depletion. The final isolated fraction contains highly purified CD4+CD127lowCD25+ cells that express high levels of FOXP3 and are immediately ready for downstream applications. An optional protocol allows for the isolation of CD4+CD25- responder T cells in parallel for use in functional studies. Following cell isolation with this EasySep™ kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins, or other chemically related ligands.
Advantages:
• Highly purified human CD4+CD127lowCD25+ Tregs isolated in less than 1 hour• No-wash removal of EasySep™ Releasable RapidSpheres™• Optional isolation of CD4+CD25- responder T cells from the same sample
Components:
- EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (Catalog #18063)
- EasySep™ Human CD25 Positive Selection Cocktail, 1 mL
- EasySep™ Human CD127high Depletion Cocktail, 1 mL
- EasySep™ Human CD4+ T Cell Enrichment Cocktail, 1 mL
- EasySep™ Releasable RapidSpheres™, 1 mL
- EasySep™ Dextran RapidSpheres™, 2 x 1 mL
- EasySep™ Release Buffer, 2 x 1 mL
- RoboSep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (Catalog #18063)
- EasySep™ Human CD25 Positive Selection Cocktail, 1 mL
- EasySep™ Human CD127high Depletion Cocktail, 1 mL
- EasySep™ Human CD4+ T Cell Enrichment Cocktail, 1 mL
- EasySep™ Releasable RapidSpheres™, 1 mL
- EasySep™ Dextran RapidSpheres™, 2 x 1 mL
- EasySep™ Release Buffer, 2 x 1 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125) x 2
- EasySep™ EasyTube™-14 (Catalog #20128)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells; T Cells, CD4+; T Cells, Regulatory
Species:
Human
Sample Source:
Leukapheresis; PBMC
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology
