MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
Members of the semaphorin protein family, such as SEMA5A, are involved in axonal guidance during neural development.
Applications: |Suitable for use in FLISA and Western Blot. Other applications not tested.
Recommended Dilution
FLISA: 1:1,000Western Blot: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
References
1. Bialecka,M., Neurosci. Lett. 399(1-2), 121-123 (2006). 2. Melin,M., Neuropsychobiology 54(1), 64-69 (2006).
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