Therecombinantproteinover-expressionsystemswithwell-knownepitopetagsarepowerfulstrategiesincheckingandpurifyingtheproteinsofinterest,whichareexpressedincertainvectorscontainingtheparticulartaggedfragments.Thesmalltagsareusedtobeclonedalongwiththetargetproteins,andtheantibodiesagainstthosefusion-tagsareusedtomonitorfusionproteinexpressionandpurification.S-Tagisanepitopetagcomposedofa15aapeptide(KETAAAKFERQHMDS)derivedfrompancreaticribonucleaseA.ThisantibodycanbeusedtocheckS-taggedexpressedproteins.

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