ImmunoTek™ TMB Stable Liquid Substrate 1.56mM for HRPO (3,3',5,5'-Tetramethylbenzidine)||TMB produces a soluble end product which is blue in color that is quantitated at 650nm. Upon addition of an equal volume of 0.5M/Liter HCl, the resulting yellow chromogen is quantitated at 450nm. The yellow product is stable at room temperature for at least 1 hour displaying less than a 1% loss in absorbance during this time interval. 1.56mM TMB is recommended for manual assays.||Benzidine and its derivatives are well documented electron donors for the horseradish peroxidase (HRP) /H2O2 oxidation/reduction system. These amines are known to be potent carcinogens.||The synthesis of TMB by Holland, et al., provided a reagent more sensitive than benzidine and much less hazardous due to ortho methylation.||Using H2O2 as substrate, the reaction of TMB with HRP proceeds through 2 phases. First, a charge transfer complex, the result of a 1-electron oxidation is formed. This is a blue product displaying an absorption maximum at 650nm. Further oxidation proceeds through a green color shift to the fully oxidized yellow diimine with an absorption maximum at 450nm.||The diimine is quantitatively formed only if the molar concentration of H2O2 is twice that of TMB. The molar extinction coefficient of the charge transfer complex is reported to be 3.9x10e4 mol-1 cm-1 and that of the diimine to be 5.9x10e4 mol-1 cm-1.The blue reaction product can be converted to the diimine non-enzymatically by the addition of acid providing an increase in sensitivity of at least 1.5.||1.56mM/Liter TMB is designed to measure low levels of HRP preventing the formation of the diimine allowing the user to determine enzyme activity or analyte concentration kinetically at 650nm or as an end point at 450nm after the addition of acid.Features:|• Single component system|• Room temperature stable|• Exhibits low background levels|• Safe to use. No weighing of hazardous powders|• Save preparation time. Use right out of the bottle|• Ultra-low sensitivity|• Minimal lot-to-lot variation||Appearance: Colorless to pale yellow/orange||Stability (18-26°C): Passes||Stability (4°C): Passes||pH: 4.9 ± 0.03||Storage and Stability: Stable for 18 months at RT. To extend the stability of product, refrigerate at 4°C.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. 2018年8月，蚂蚁淘成为UnitedStatesBiological（简称USBio）代理商，UnitedStatesBiological也简称为USBiological。 欢迎广大客户咨询订货，在线QQ：3507623898 4000-520-616 最主要的一抗产品： 144747-FITC Anti-IL-6(BCellstimulatoryfactor2)(FITC) Pab rabbit human IgG Hu FITC $592 IL6 WB 100ul 144764-FITC Anti-Interferongamma(IFN-gamma)(FITC) Pab rabbit rat IgG Rt FITC $592 IFNG E,IHC,IP,N,WB 100ul 144739-FITC Anti-IL-3(Hematopoieticgrowthfactor,Mastcellgrowthfactor)(FITC) Pab rabbit human IgG Hu FITC $592 IL3 E,IHC,IP,N,WB 100ul 144964-FITC Anti-NFKB1(KBF1,NF-kappaB,NFkappaB,NF-kB1,NFKB-p50,EBP-1,p50,p105,TRANSCRIPTIONFACTORNFKB1,DNA-bindingfactorKBF1,NFKBp50,INCLUDED,NFKBp105,INCLUDED,NuclearfactorofkappalightpolypeptidegeneenhancerinBCells1)(FITC) Pab rabbit human IgG Hu,Mo,Rt FITC $628 NFKB1 WB 100ul 303092-FITC Anti-ACE(ACE1,ACET,ACE_HUMAN,ACE1,AngiotensinConvertingEnzymeSomaticIsoform,AngiotensinConvertingEnzymeTestisSpecificIsoform,AngiotensinIConvertingEnzyme,AngiotensinIConvertingEnzyme1,AngiotensinIConvertingEnzymePeptidylDipeptidaseA1,Angiotensin-convertingEnzyme,Carboxycathepsin,CD143,CD143,CD143Antigen,DCP1,DCP,DCP1,DipeptidylCarboxypeptidase1,DipeptidylCarboxypeptidaseI,KininaseII,MVCD3,PeptidaseP,PeptidylDipeptidaseA,SolubleForm,TesticularECA)(FITC) Pab rabbit human IgG Hu,Mo,Rt FITC $592 IHC,WB 100ul 145142-FITC Anti-SCF(Mastcellgrowthfactor)(FITC) Pab rabbit human IgG Hu FITC $592 KITLG E,WB 100ul 145147-FITC Anti-SDHA(FlavoproteinsubunitofcomplexII)(FITC) Pab rabbit human IgG Hu,Mo,Rt FITC $628 SDHA IHC,WB 100ul 145286-FITC Anti-TNC(Hxb,Hexabrachion,Tenascin-C,TN-C,TN,CYTOTACTIN,hexabrachion(tenascinC,cytotactin),hexabrachion(tenascin),MGC167029)(FITC) Pab rabbit human IgG Hu,Mo,Rt FITC $628 TNC IHC,WB 100ul 303394-FITC Anti-Interleukin1Beta(IL1beta,Catabolin,H1,Hematopoietin1,IFNbetaInducingFactor,IL1,IL1beta,IL1B,IL-1beta,IL1Beta,IL1B,IL1B_HUMAN,IL1F2,Interleukin1betaPrecursor,Interleukin-1beta,LAF,OAF,OsteoclastActivatingFactor,Preinterleukin1beta,Preinterleukinbeta,ProInterleukin1beta)(FITC) Pab rabbit rat IgG Rt FITC $592 E,WB 100ul 303592-FITC Anti-SerumAmyloidP(9.5Salpha1Glycoprotein,9.5Salpha-1-glycoprotein,AmyloidPComponentSerum,APCS,PentaxinRelated,Pentraxin2,PTX2,PTX2,SAMP_HUMAN,SAP,SerumAmyloidPComponent,SerumAmyloidP-component(1-203))(FITC) Pab rabbit rat IgG Rt FITC $592 IHC,WB 100ul 144120-HRP Anti-Actin(Gamma-actin)(HRP) Pab rabbit human IgG Hu,Mo,Rt HRP $628 ACTG1 IHC,WB 100ul 144129-HRP Anti-ADAMTS2(ProcollagenIN-proteinase)(HRP) Pab rabbit human IgG Hu,Rt HRP $628 ADAMTS2 IHC,WB 100ul 144160-HRP Anti-ANGPTL3(ANG-5,ANGPT5,Angiopoietin-5)(HRP) Pab rabbit human IgG Hu,Mo,Rt HRP $628 ANGPTL3 WB 100ul 144183-HRP Anti-ApoptosisregulatorBcl-2(HRP) Pab rabbit human IgG Hu HRP $592 BCL2 E,IP,N,WB 100ul 144190-HRP Anti-AQP3(Aquaglyceroporin-3)(HRP) Pab rabbit human IgG Hu,Mo,Rt HRP $628 AQP3 IC,IHC,WB 100ul