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蚂蚁淘在线 / 品牌中心 / Usbio / Usbio/381868 AKAP12 (A Kinase Anchor Protein 12) BioAssay™ ELISA Kit (Human)/381868/96Tests
商品描述
SampleType
Serum,Plasma,BIOLOGicalFluids
IntendedUse
ThisBioAssay™kitisasandwichELISAforinvitroquantitativemeasurementofAKAP12inhumanserum,plasmaandotherbiologicalfluids
Sensitivity
9.375pg/mL
Range
15.625-1000pg/mL
Specificity
Human
TestPrinciple
ThisBioAssay™ELISAkitusesSandwich-ELISAasthemethod.ThemicroELISAplateprovidedinthiskithasbeenpre-coatedwithanantibodyspecifictohumanAKAP12(AKinaseAnchorProtein12).StandardsorsamplesareaddedtotheappropriatemicroELISAplatewellsandcombinedwiththespecificantibody.ThenabiotinylateddetectionantibodyspecificforhumanAKAP12(AKinaseAnchorProtein12)andAvidin-HorserADIshPeroxidase(HRP)conjugateareaddedtoeachmicroplatewellsuccessivelyandincubated.Freecomponentsarewashedaway.Thesubstratesolutionisaddedtoeachwell.OnlythosewellsthatcontainhumanAKAP12(AKinaseAnchorProtein12),biotinylateddetectionantibodyandAvidin-HRPconjugatewillappearblueincolor.Theenzyme-substratereactionisterminatedbyaddingStopSolutionandthecolorturnsyellow.Theopticaldensity(OD)ismeasuredspectrophotometricallyatawavelengthof450nm±2nm.TheODvalueisproportionaltotheconcentrationofhumanAKAP12(AKinaseAnchorProtein12).TheconcentrationofhumanAKAP12(AKinaseAnchorProtein12)insamplescanbecalculatedbycomparingtheODofthesamplestothestandardcurve.
KitComponents
MicrotiterStrips,8x12wellsStandard,2vialsSample/StandardDiluentBuffer,1x20mlAntibody-Biotin,1x120ulAntibody-BiotinDiluent,1x10mlHRPConjugate,1x120ulHRPConjugateDiluent,1x10mlSubstrateReagent,1x10mlStopSolution,1x10mlWashBuffer(25X),1x30ml
StorageandStABIlity
Unusedkitisstablefor6monthsafterreceipt. StoreMicrotiterStrips,Standard,andAntibody-Biotinat4°Cifkitwillbeusedwithin30days;otherwisestoreat-20°Cuntilexpirationdate.Storetherestofthecomponentsat4°C.ProtectHRPConjugateandSubstrateReagentfromlight.Formaximumrecovery,centrifugetheoriginalvialsafterthawingandpriortoremovingthecap.


