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蚂蚁淘在线 / 品牌中心 / Signagen / Signagen/shRNA/miRNA/gRNA Adenovirus Production Full Service Pilot Scale/SL100785/

Signagen/shRNA/miRNA/gRNA Adenovirus Production Full Service Pilot Scale/SL100785/

价格
面议
货号：SL100785
浏览量：127
品牌：Signagen
服务
全国联保
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商品描述
shRNA/miRNA/gRNAAdenovirusProductionFullServicePilotScale

ProductionofRecombinantSEROtype5shRNA/miRNAAdenovirus(E1/E3deletion)withAd.MAXSystem:
-One-stopservice:fromshRNA/miRNAdesigntopackaging.
-Unsurpassabletransductionefficiency.
-Quickturnaroundtime:3to4weeks.
-Cost-effective.
-Specialdesignrequestacceptable,FREE!

LargeshRNA/miRNA/gRNAAd.MAX™shuttlevectorcollectionforcloningshuttleplasmid with aspecificpromoterandreporter:

 Single-promoterAd.MAX™shuttlevector

 Dual-promoterAd.MAX™shuttlevector

           Promoterless
           CMV
           CAG
           H1
           U6
           Synapsin
           UBC
           EF1α
           ALB(1.4)
           ApoE/AAT1
           CaMKII
           ELA1
           Enh358MCK
           cTNT
           GFAP
           MBP
           SST
           TBG
           αMHC
           hRPE
           mIP1
           tMCK



















 

           Promoterless
           CMV
           CAG
           H1
           U6
           Synapsin
           UBC
           EF1α
           ALB(1.4)
           ApoE/AAT1
           CaMKII
           ELA1
           Enh358MCK
           cTNT
           GFAP
           MBP
           SST
           TBG
           αMHC
           hRPE
           mIP1
           tMCK

CMV
CAG
H1
U6
Synapsin
UBC
EF1α
CaMKII
cTNT
GFAP











 

eGFP
RFP
mRFP
mCherry
tdTOMATO
TurboGFP
eYFP
Venus
Luc
LacZ











 


ServiceDescription:
1.SynthesizeandcloneshRNA/miRNAtoashuttlevectorfollowedbytransferofthewholetransgenecassettetoadenoviralgenome.
2.ViralproductioninAd.MAX™293cells:transfectionandpurificationofrecombinantadenovirus.
3.Generationofhightiterrecombinantadenoviralstockin~500cm2Ad.MAX™293cells.
4.RecombinantadenovirusPFUtitration.

RequiredMaterials:NCBIaccession#oftargetgene,targetregionsequence,shRNAorsiRNAsequencevalidated/publishedormaturemiRNAsequence,miRNAaccession#orgRNAsequenceetc.

TurnaroundTime:3~4weeks.

Deliverables:1.0mL(5x0.2mL)ofsuperinfectiousinvitrogradeadenovirusvectorat5E+10~5E+11PFU/mL*.

Weofferdiscountfornewcustomer,pleaserequestaquotewithustoday.

WealsooffertruncatedcustomshRNA/miRNA/gRNAadenovirusservice,pleaserequestaquotewithustoday.

*Finalviralyieldmaydependonthetransgeneinthevirus.
 

Signagen腺伴随病毒（AAV）是一种小型（直径20 nm）的复制缺陷型无包膜病毒，可感染人类和其他灵长类动物。目前尚不知道AAV会引起疾病，因此该病毒会引起非常轻微的免疫反应。AAV可以感染分裂细胞和非分裂细胞，并且可以将其基因组整合到宿主细胞的基因组中。