Signagen/shRNA/miRNA/gRNA Adenovirus Production Full Service Large Scale/SL100782/

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货号:SL100782
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品牌:Signagen
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商品描述
shRNA/miRNA/gRNAAdenovirusProductionFullServiceLargeScale

ProductionofRecombinantSEROtype5shRNA/miRNA/gRNAAdenovirus(E1/E3deletion)withAd.MAXSystem:
-One-stopservice:fromshRNA/miRNAdesigntopackaging.
-Unsurpassabletransductionefficiency.
-Quickturnaroundtime:3to4weeks.
-Cost-effective.
-Specialdesignrequestacceptable,FREE!


LargeshRNA/miRNA/gRNAAd.MAX™shuttlevectorcollectionforcloningshuttleplasmid with aspecificpromoterandreporter:

 Single-promoterAd.MAX™shuttlevector

 Dual-promoterAd.MAX™shuttlevector

     Promoterless
     CMV
     CAG
     H1
     
U6
     Synapsin
     UBC

     EF1α
     ALB(1.4)
     ApoE/AAT1
     CaMKII
     ELA1
     Enh358MCK
     cTNT
     GFAP
     MBP
     SST
     TBG

     αMHC
     hRPE
     mIP1
     tMCK



















 

     Promoterless
     CMV
     CAG
     H1
     
U6
     Synapsin
     UBC

     EF1α
     ALB(1.4)
     ApoE/AAT1
     CaMKII
     ELA1
     Enh358MCK
     cTNT
     GFAP
     MBP
     SST
     TBG

     αMHC
     hRPE
     mIP1
     tMCK

CMV
CAG
H1

U6
Synapsin
UBC

EF1α
CaMKII
cTNT
GFAP











 

eGFP
RFP
mRFP
mCherry
tdTOMATO
TurboGFP
eYFP
Venus
Luc
LacZ











 


ServiceDescription:
1.Design,synthesizeandcloneshRNA/miRNA/gRNAtoashuttlevectorfollowedbytransferofthewholetransgenecassettetoadenoviralgenome.
2.Initialadenoviralstockproduction.
3.Adenovirusamplificationfrom
~4000cm2Ad.MAX™293cells.
4.Adenoviruspurificationvia2xCsClultracentrifugation.
5.RecombinantadenovirusPFUtitration.


RequiredMaterials:NCBIaccession#oftargetgene,targetregionsequence,shRNAorsiRNAsequencevalidated/publishedormaturemiRNAsequence,miRNAaccession#orgRNAsequenceetc.

TurnaroundTime:3~4weeks.

Deliverables:2mL(10x0.2mL)ofsuperpurifiedinvivogradeadenovirusvectorat5E+10~5E+11PFU/mL*.

Weofferdiscountfornewcustomer,pleaserequestaquotewithustoday.

WealsooffertruncatedshRNA/miRNA/gRNAservice,please
requestaquotewithustoday.


*Finaladenovirusyieldmaydependonthenatureoftransgene.

Signagen腺伴随病毒(AAV)是一种小型(直径20 nm)的复制缺陷型无包膜病毒,可感染人类和其他灵长类动物。目前尚不知道AAV会引起疾病,因此该病毒会引起非常轻微的免疫反应。AAV可以感染分裂细胞和非分裂细胞,并且可以将其基因组整合到宿主细胞的基因组中。