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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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商品描述
shRNAValidationServiceviaCell-basedReporterSystem:
-CloningORFofgeneofinterests(GOI)intoanadenovirusshuttlevectorwithareporter.
-Designandsynthesize~6shRNAoligoesfollowedbycloningthemtotheaboveshuttleconstructtoobtainshRNAvalidationplasmid.
-TransienttransfectiontheshRNAvalidationplasmid.
-QuantificationoftargetgenesilencingviaFACS(forGFPexpression)orluminometer(forluciferaseexpression).
AvailableshRNAValidationPlatforms:
-ConstitutiveshRNAknockdown
-InducIBLeshRNAknockdown
GuaranteedKnockdown:
Weguaranteeatleast1shRNAat>80%knockdownofmRNAlevelinthetestedcellsbyscreening~6shRNAoligoes.
Weofferdiscountfornewcustomer,please requestaquotewithustoday.
Figure1. ACartoonShowingshRNAValidationProcessforshRNAAdenovirusProduction.
