Signagen/LumiGOLD™ ECL Western Blotting Detection Kit (Ver. II)/SL100309/250 mL

价格
¥10520.00
货号:SL100309-1000mL
浏览量:127
品牌:Signagen
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商品描述

Application:SubstrateofHorserADIshperoxidaseforchemiluminescentdetectionofproteininWesternBlot

Features:

▪Chemiluminescencelastsforupto6hourswithoutsignificantattenuation
▪Extremelysensitiveabletodetect0.1pgproteinwithverylowbackground
▪Extremelystableforstorageupto12mouthsat4°C
▪Workingsolutionstableupto72hoursat4°C

ComparisonsofLumiGOLD™ECLWesternBlottingDetectionKitwithOtherLeadingBrandProducts:
A.LumiGOLD™ECLWesternBlottingDetectionofEGFP(enhancedgreenfluorescentprotein)inWideLinearRange*




*10two-folderserialdilutionsofpurifiedEGFP(0.1pg~51.2pginlane1~10fromlefttoright,upperpanel).EGFPwasseparatedbySDS-PAGEelectrophoresisfollowedbytransfertoaPVDFmembrane.Themembranewasblockedwith5%skimmilk(product#SL100317)andwashedwithTBSTfollowedbyincubationofmouseanti-EGFPmonoclonalantibody.HRP-conjugatedgoatanti-mouseIgGwasusedtorecognizethemouseanti-GFP.TheblotwasexposedtoFujifilmfor20seconds.ThelinearrangeofLumiGOLDD™ECLkitwasdetermined(lowerpanel)byadditionof10two-folderserialdilutedhorseradishperoxidase(HRP)totheLumiGOLD™workingsolutionfollowedbydeterminationofluminositybyBeckmanCoulterLD400luminometer


B.AcomparisonofLumiGOLD™ECLWesternBlottingDetectionKitwithPopularBrandProducts

Products

Noise*

Signal**

Signal/Noise

LumiGOLD™

18905

1938181

103

AmershamECL™

1495

93098

62

AmershamECL™Plus

320478

3480213

10.9

PiercePicoFemto™

362103

3353736

9.3

*Noisereferstothebackgroundchemiluminescenceoftheworkingsolutionwithoutadditionofhorseradishperoxidase(HRP)
**SignalreferstotheHRPcatalyzedchemiluminescenceoftheworkingsolutionafteradditionof5µ
lxHRP-conjugatedgoatanti-mouseIgG(100,000dilution).LuminositywasdeterminedbyaBeckmanCoulterLD400luminometer

C.LumiGOLD™ECLWesternBlottingDetectionofbeta-ActininComparisonofAmershamECLKit*

          AmershamECL                  LumiGOLD™



*HEK293celllysatewaspreparedwithCompLysis™ProteinExtractionReagent(Cat#SL100319)andthesameamountoftotalproteinswereloadedtothecorrespondingwells(lane#1=lane#5/lane#2=lane#6/lane#3=lane#7/lane#4=lane#8).beta-Actinwaspickedupbyanti-actinantibodyfollowedbydevelopmentwithECLreagentsfor2minutes.Theexposuretimewaslabeledintheaboveimages

DataSheet

Testimonials
Ihaveuseditforseveraltimes,itisgoodaccordingtotheresults.Itseemsthatthisproductisbetterthanotherstosomedegree...
------byDr.AibingWang,NHLBI,NIH

Signagen腺伴随病毒(AAV)是一种小型(直径20 nm)的复制缺陷型无包膜病毒,可感染人类和其他灵长类动物。目前尚不知道AAV会引起疾病,因此该病毒会引起非常轻微的免疫反应。AAV可以感染分裂细胞和非分裂细胞,并且可以将其基因组整合到宿主细胞的基因组中。
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