Description:
HapiCellTransfectionToxicityRemovalCocktailwasourproprietaryformulationforeffectivelyeliminatingDNAorsiRNAtransfectionmediatedtoxicity.HapiCellTransfectionToxicityRemovalCocktailwasdevelopedtocontainamixtureofseveralchemicalswhichwereconfirmedtoeffectivelyremoveDNA/siRNAtransfectionmediatedcytotoxicity.Incubationofsensitivecells(e.g.,SaoS-2,HUVEC,primarykerotinocytes,etc)withHapiCellToxicityRemovalReagentfor2minutesatRTwasconfirmtocompletelyremovethetransfectionrelatedtoxicitywhichotherwisekillthecells.Thecocktailwasusedforchemicaltransfectiononly,notforelectroporation.HapiCellTransfectionToxicityRemovalCocktail,2.5ml(x50stocksolution),issufficienttotreat125wellsfor24-wellplateand63wellsfor6-wellplaterespectively.
Feature:
-Removestransfectionmediatedtoxicityafter2minutesincubationatRT
-Nontoxictocells
-MaintaincellviABIlity
-Improvetransfectionefficiency
-Easytouse
HapiCellTransfectionToxicityRemovalCocktailEffectivelyRemoveTransfectionMediatedToxocity:
AexampleshowingcompleteremovaloftransfectiontoxicityaftertreatmentwithHapiCellTransfectionToxicityRemovalCocktailonmouseskinfibroblast.ADNAencodingGFPwasdeliveredtomouseskinfibroblastsbyLipofectamine2000inabsenceofserum.Serum-containingmediumwaschangedback5hoursposttransfectionfollowed2minutesincubationwith(leftpanel)orwithout(rightpanel)HapiCellTransfectionToxicityRemovalCocktail.Thecellviabilitywascheckedundermicroscope24hoursposttransfection
AexampleshowingcompleteremovaloftransfectiontoxicityaftertreatmentwithHapiCellTransfectionToxicityRemovalCocktailonprimarymousekerotinocytes.ADNAencodingGFPwasdeliveredtoprimarymousekerotinocytesbyLipofectamine2000inabsenceofserum.Serum-containingmediumwaschangedback5hoursposttransfectionfollowed2minutesincubationwith(leftpanel)orwithout(rightpanel)HapiCellTransfectionToxicityRemovalCocktail.Thecellviabilitywascheckedundermicroscope24hoursposttransfection
DataSheet
