Description:
HapiCellTransfectionToxicityRemovalCocktailwasourproprietaryformulationforeffectivelyeliminatingDNAorsiRNAtransfectionmediatedtoxicity. HapiCellTransfectionToxicityRemovalCocktailwasdevelopedtocontainamixtureofseveralchemicalswhichwereconfirmedtoeffectivelyremoveDNA/siRNAtransfectionmediatedcytotoxicity. Incubationofsensitivecells(e.g.,SaoS-2,HUVEC,primarykerotinocytes,etc)withHapiCellToxicityRemovalReagentfor2minutesatRTwasconfirmtocompletelyremovethetransfectionrelatedtoxicitywhichotherwisekillthecells. Thecocktailwasusedforchemicaltransfectiononly,notforelectroporation. HapiCellTransfectionToxicityRemovalCocktail,125ml,issufficienttotreat125wellsfor24-wellplateand63wellsfor6-wellplaterespectively.
Feature:
-Removestransfectionmediatedtoxicityafter2minutesincubationatRT
-Nontoxictocells
-MaintaincellviABIlity
-Improvetransfectionefficiency
-Easytouse
HapiCellTransfectionToxicityRemovalCocktailEffectivelyRemoveTransfectionMediatedToxocity:
AexampleshowingcompleteremovaloftransfectiontoxicityaftertreatmentwithHapiCellTransfectionToxicityRemovalCocktailonmouseskinfibroblast.ADNAencodingGFPwasdeliveredtomouseskinfibroblastsbyLipofectamine2000inabsenceofserum.Serum-containingmediumwaschangedback5hoursposttransfectionfollowed2minutesincubationwith(rightpanel)orwithout(leftpanel)HapiCellTransfectionToxicityRemovalCocktail.Thecellviabilitywascheckedundermicroscope24hoursposttransfection
AexampleshowingcompleteremovaloftransfectiontoxicityaftertreatmentwithHapiCellTransfectionToxicityRemovalCocktailonprimarymousekerotinocytes.ADNAencodingGFPwasdeliveredtoprimarymousekerotinocytesbyLipofectamine2000inabsenceofserum.Serum-containingmediumwaschangedback5hoursposttransfectionfollowed2minutesincubationwith(rightpanel)orwithout(leftpanel)HapiCellTransfectionToxicityRemovalCocktail.Thecellviabilitywascheckedundermicroscope24hoursposttransfection
DataSheet
