Description
GenJetInVitroDNATransfectionReagent(Ver.II)isupgradedversionofGenJetInVitroDNATransfectionReagent.Adoptinganewchemistry,moreDNAcondensinggroupswereliberatedinGenJet(Ver.II)(100%vs.80%inoldversion),leADIngto3~4foldsincreaseoftransfectionefficiencywithoutsacrificingcellviABIlity.GenJet(Ver.II)reagent,1.0ml,issufficientfor~667transfectionsin24wellplatesor~333transfectionsin6wellplates.
FeaturescomparedwitholdversionGenJetInVitroDNATrasnfectionReagent
-Cell-dependent3~4timeshigherefficiency
-Topchoiceforhard-to-transfectcells
-Efficiencyboostedinthepresenceofserumandantibioticsformostofcelltypes
-Exceptionalhighlevelsofrecombinantproteinproduction
StorageCondition
Storeat4°C.Ifstoredproperly,theproductisstablefor12monthsorlonger.
ComparisonsofTransfectionEfficiencyandCytotoxicityofGenJet(Ver.II)withOldVersionGenJetandOtherLeadingProducts
Comparisonoftransfectionefficiency(leftpanel)andtoxicity(rightpanel)GenJetVer.IIwitholdversionGenJetoncommonlyusedmammaliancells.phRL-CMVencodingRenillaLuciferasewasintroducedintocellswiththepresenceofserumandantibiotics.TheRenilliaLuciferaseactivitywasdeterminedwithRenillaLuciferaaseAssaySystem(Promega).Forcytotoxicity,MTTassaywasemployedtodeterminecellviabilitywithuntransfectedcontrolas100%.
AcomparisonoftransfectionefficiencyofGenJet(Ver.II)vs.Lipofectamine2000onprimaryrataorticsmoothmusclecells.PrimaryrataorticsmoothmusclecellswerepreparedandtransfectedwithanucleartargetedGFPCDNAbyGenJet(rightpanel)andLipofectamine2000(L2K,leftpanel)respectively.ThetransfectionefficiencywerecheckedunderaNikonEclipse2000fluorescencemicroscopy48hoursposttransfection
UpperPanel:TransfectionefficiencycomparisonofGenJetVer.IIwitholdversionGenJeton293Tcells.pEGFP-N3wastransfectedto293Tcellsat90%confluencywithpresenceofserumandantibiotics.GFPfluorescencewasdetectedwithNikonEclipse2000microscopy48hoursposttransfection
Lowerpanel:TransfectionefficiencycomparisonofGenJetVer.IIwitholdversionGenJetonHelacells.pEGFP-N3wastransfectedtoHelacellsat90%confluencywithpresenceofserumandantibiotics.GFPfluorescencewasdetectedwithNikonEclipse2000microscopy48hoursposttransfection
TechnicalInformation&Datasheet
-ACompleteTransfectionProtocol
-AShortProtocolforAdvancedUsers
-AProtocolforTransfectingSUSPension293andCHOCells
- AProtocolforLentivirusProduction
- AProtocolrAAVProduction
-TechnicalNote&TransfectionTips
RelatedProduct:GenJetInVitroDNATransfectionreagent
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