Signagen/GenJet™ In Vitro DNA Transfection Kit for HUVEC/SL100489-HUVEC/Trial kit (1x0.1 mL)

价格
¥35040.00
货号:SL100489-HUVEC-5kits
浏览量:127
品牌:Signagen
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商品描述

ContentsPerKit:
1x1.0mlofGenJet™DNATransfectionReagent
●1x8.0ml(x5)ofGenJetll™TransfectionBuffer

Description

GenJet™DNAInVitroTransfectionReagentforHUV
ECispre-optimizedfortransfectinghumanumbilicalveinendothelialcells(HUVEC). HUVECisoneofhard-to-transfectcelllinespartlyduetothetransfectiontoxicityrelatedcelldeath.IncombinationofGenJet™TransfectionBuffer,thepre-optimizedtransfectionreagentgaveupto60%efficiency.RefertothefollowingoptimaltransfectionconditionsformaximaltransfectionefficiencyonHUVEC.GenJet™reagent,1.0ml,issufficientfor500transfectionsin24wellplatesor250transfectionsin6wellplates.

SummaryofOptimalShavedCellTransfectionConditions:
Cellculturemedium
Confluencyofcellonthedayofshaving
Celldensityforplating

GenJet™(µl):DNA(µg)Ratio
DiluentforDNAandTransfectionReagent

IncubationTimetoFormGenJet™/DNAComplex
MaximalEfficiency

TransfectionResults:
ReporterGene
Plasmid

Efficiency(GFP%)


DMEMwith4.5g/Lglucose,10%FBS
70%
1.3x10^5cellspersquarec
entimeter
2.25:1

GenJet™TransfectionBuffer(1x)
10minutesatRT
48hours


EGFP
pEGFP-N3(CMVpromoter)
56%

StorageCondition
Storeat40C.Ifstoredproperly,theproductisstablefor12monthsorlonger

DataSheet

Signagen腺伴随病毒(AAV)是一种小型(直径20 nm)的复制缺陷型无包膜病毒,可感染人类和其他灵长类动物。目前尚不知道AAV会引起疾病,因此该病毒会引起非常轻微的免疫反应。AAV可以感染分裂细胞和非分裂细胞,并且可以将其基因组整合到宿主细胞的基因组中。
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