Signagen/Custom Lentivirus Production Full Service, Pilot Scale/SL100201/

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货号:SL100201
浏览量:127
品牌:Signagen
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商品描述

ProductionofRecombinantPseudotypedLentiviruswithLenti.MAXPackagingSystem:
-Derivedfrom3rdgenerationpackagingsystemwithashRNAconstruct includedformaximumyield. 
-Aninhibitorytranselementupstreamof5'-LTRallowspackagingtoxictransgenes.
-LentiMAX™packagingcelllineisoptimizedforhighpseudoviraltiters.

Large lentivirustransvectorinventoryforconstructinglentivectorcarryingaspecificpromoterandreporter:


ServiceDescription:
1.Cloneyourgeneofinterest(GOI)intoalentivirustransvector.
2.LentiviruspackagingviaLenti.MAX
™packagingsystem..
3.LentiviruspurificationviaPEGprecipitationfollowedbyultra-centrifugation.

4.Recombinantlentivirustitrationviap24ELISA.

RequiredMaterial:ANCBIaccession#orgenesymbolorplasmidDNAcarryingyourgeneofinterest(GOI).

TurnaroundTime:~2weeks.

Deliverables:>0.25mL(2x125
µL)at>10^9TU/mL*invivograde**superpurifiedlentiviralvector.

Weofferdiscountfornewcustomer,pleaserequestaquotewithustoday.

Weoffertruncated serviceaswell,pleaserequestaquotewithustoday.


*Despitethefactthatthefinallentivirusyielddependsonthenatureoftransgeneandvariesfrombatchtobatch,weguaranteethetiter>10^9TU/mL.
**
WedeliverinvivogradeoflentiviruswhichisOKforbothinvitrotissuecultureinfectionandinvivoinjection.

Signagen腺伴随病毒(AAV)是一种小型(直径20 nm)的复制缺陷型无包膜病毒,可感染人类和其他灵长类动物。目前尚不知道AAV会引起疾病,因此该病毒会引起非常轻微的免疫反应。AAV可以感染分裂细胞和非分裂细胞,并且可以将其基因组整合到宿主细胞的基因组中。