ProductionofRecombinantPseudotypedLentiviruswithLenti.MAXPackagingSystem:
-Derivedfrom3rdgenerationpackagingsystemwithashRNAconstruct includedformaximumyield.
-Aninhibitorytranselementupstreamof5'-LTRallowspackagingtoxictransgenes.
-LentiMAX™packagingcelllineisoptimizedforhighpseudoviraltiters.
Large lentivirustransvectorinventoryforconstructinglentivectorcarryingaspecificpromoterandreporter:
ServiceDescription:
1.Cloneyourgeneofinterest(GOI)intoalentivirustransvector.
2.LentiviruspackagingviaLenti.MAX™packagingsystem..
3.LentiviruspurificationviaPEGprecipitationfollowedbyultra-centrifugation.
4.Recombinantlentivirustitrationviap24ELISA.
RequiredMaterial:ANCBIaccession#orgenesymbolorplasmidDNAcarryingyourgeneofinterest(GOI).
TurnaroundTime:~2weeks.
Deliverables:>1.0mL(5x0.2mL)at>10^9TU/mL*invivograde**superpurified lentiviralvector.
Weofferdiscountfornewcustomer,pleaserequestaquotewithustoday.
Weoffertruncatedserviceaswell,pleaserequestaquotewithustoday.
*Despitethefactthatthefinallentivirusyielddependsonthenatureoftransgeneandvariesfrombatchtobatch,weguaranteethetiter>10^9TU/mL.
**WedeliverinvivogradeoflentiviruswhichisOKforbothinvitrotissuecultureinfectionandinvivoinjection.