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蚂蚁淘在线 / 品牌中心 / Signagen / Signagen/Custom Adenovirus Production Full Service, Pilot Scale/SL100736/

Signagen/Custom Adenovirus Production Full Service, Pilot Scale/SL100736/

价格
面议
货号：SL100736
浏览量：127
品牌：Signagen
服务
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商品描述
CustomAdenovirusProductionFullService,PilotScale

ProductionofRecombinantSEROtype5Adenovirus(E1/E3deletion)withAd.MAXSystem:
-Unsurpassabletransductionefficiency(nearly100%vs.AAV30~40%andLentivirus~30%)inmostmammaliancells.
-Nointegrationintohostgenome.
-CompatIBLewithtoxicgeneofinterest(GOI).
-Inducehigh-leveltransientproteinexpression.
-Accommodateinsertsofupto7.5kb.

LargecollectionofAd.MAX™shuttlevectorforcloningshuttleplasmid with aspecificpromoterandreporter:

SinglepromoterAd.MAX™shuttlevector

DualpromoterAd.MAX™shuttlevector

             Promoterless
             CMV
             CAG
             H1
             U6
             Synapsin
             UBC
             EF1α
             ALB(1.4)
             ApoE/AAT1
             CaMKII
             ELA1
             Enh358MCK
             cTNT
             GFAP
             MBP
             SST
             TBG
             αMHC
             hRPE
             mIP1
             tMCK



















 

              Promoterless
              CMV
              CAG
              H1
              U6
              Synapsin
              UBC
              EF1α
              ALB(1.4)
              ApoE/AAT1
              CaMKII
              ELA1
              Enh358MCK
              cTNT
              GFAP
              MBP
              SST
              TBG
              αMHC
              hRPE
              mIP1
              tMCK

Promoterless
CMV
CAG
H1
U6
Synapsin
UBC
EF1α
CaMKII
cTNT
GFAP










 

eGFP
RFP
mRFP
mCherry
tdTOMATO
TurboGFP
eYFP
Venus
Luc
LacZ











 


ServiceDescription:
1.Cloneyourgeneofinterest(GOI)intoashuttlevectorfollowedbytransferofthewholetransgenecassettetoadenoviralgenome.
2.Initialadenoviralstockproduction.
3.Adenovirusamplificationfrom~500cm2Ad.MAX™293cells.
4.RecombinantadenovirusPFUtitration.

RequiredMaterial:ANCBIaccession#orgenesymbolorplasmidDNAcarryingyourgeneofinterest(GOI).

TurnaroundTime:3~4weeks.

Deliverables:>1.0mL(5x0.2mL)superinfectiousinvitrogradeofadenovirusvectorat5E+10~5E+11PFU/mL*.

Weofferdiscountfornewcustomers,pleaserequestaquotewithustoday.

Weoffertruncatedserviceaswell,pleaserequestaquotewithustoday.


*Finaladenovirusyieldmaydependonthenatureoftransgene.

Signagen腺伴随病毒（AAV）是一种小型（直径20 nm）的复制缺陷型无包膜病毒，可感染人类和其他灵长类动物。目前尚不知道AAV会引起疾病，因此该病毒会引起非常轻微的免疫反应。AAV可以感染分裂细胞和非分裂细胞，并且可以将其基因组整合到宿主细胞的基因组中。