ProductionofRecombinantSEROtype5Adenovirus(E1/E3deletion)withAd.MAXSystem:
-Unsurpassabletransductionefficiency(nearly100%vs.AAV30~40%andLentivirus~30%)inmostmammaliancells.
-Nointegrationintohostgenome.
-CompatIBLewithtoxicgeneofinterest(GOI).
-Inducehigh-leveltransientproteinexpression.
-Accommodateinsertsofupto7.5kb.
LargecollectionofAd.MAX™shuttlevectorforcloningshuttleplasmid with aspecificpromoterandreporter:
SinglepromoterAd.MAX™shuttlevector | DualpromoterAd.MAX™shuttlevector | |||
Promoterless |
| Promoterless | Promoterless | eGFP |
ServiceDescription:
1.Cloneyourgeneofinterest(GOI)intoashuttlevectorfollowedbytransferofthewholetransgenecassettetoadenoviralgenome.
2.Obtaininitialadenoviralstock.
3.Adenovirusamplificationfrom~1400cm2Ad.MAX™293cells.
4.RecombinantadenovirusPFUtitration.
RequiredMaterial:ANCBIaccession#orgenesymbolorplasmidDNAcarryingyourgeneofinterest(GOI).
TurnaroundTime:3~4weeks.
Deliverables:>2.0mL(10x0.2mL)superinfectiousinvitrogradeofadenovirusvectorat>5E+10~5E+11PFU/mL*.
Weofferdiscountfornewcustomers,pleaserequestaquotewithustoday.
Weoffertruncatedserviceaswell,pleaserequestaquotewithustoday.
*Finaladenovirusyieldmaydependonthenatureoftransgene.