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Application:MamallianCellProteinExtractionforWesternBlotandOtherProteinExtractionApplications
FEATURES:
-Non-ionicdetergentformulation,suitableforBradfordandBCAProteinAssays
-Keeptheextractedproteinundenatured,suitableforanyaffinitypurificationprocedureslikeimmunoprecipitation
-KeepGST,luciferase,beta-galactosidase,CATandotherreporter/taggeneactivities
ComparisonofCompLysisStrippingReagentwiththefreeze/thawcycle,sonicationandtheMostPopularBrandProductM:
*HEK293cellsgrownin10cmplatesatfullconfluency.CompLysisReagentisutilizedtoextracttotalprotein.Forß-galactosidaseassay,HEK293cellsweretransfectedwithreporterconstructexpressingß-galactosidase.
DataSheet