ProductCategory: Pre-maderecombinantAAV;AAV-hSynapsin-DIO-GFP;AAV-Synapsin-DIO-GFP;AAV2-hSyn-DIO-GFP;AAV2-Syn-DIO-GFP
Description:
AAV2-hSyn-DIO-GFP isapre-maderAAVinSEROtype2whichwillCredependentlyover-expressGFPdrivenunderhumansynapsinpromoterasillustratedasthecartoonbelow.
GFPisagreenmonomerwhichmaturesextremelybright,makingitpossIBLetoseeresultsverysoonafteractivatingtranscription.Itishighlyphotostableandresistanttophotobleaching. IntheDIOscenario,thetransgeneofinterestisinsertedinreverseorientationrelativetothe5"promoterandisflankedbyoppositelyorientedloxPandlox2272sites. IntheabsenceofCreexpression,thetransgenewillnotbeproduced.InthepresenceofCreexpression,thetransgenewillbe"Flip-exchanged"orFLEXed,leADIngtotheexpressionofthetransgene.ThisisduetoapermanentCre-mediatedrecombination/inversionoftheflankedtransgene.ThisarrangementiscalledDIO(double-floxedinverseORF),Cre-ON,Flex-rev(reverse),Flex-ON/FlexON,orDIO-AAV/AAV-DIO(double-floxedinverseORFinAAV). 
Titer: >1x1013 VG/mL
Size: >30 µL (StdPack)
StorageBuffer: SterilePBS
InfectionProtocol: 
VectorMap:
