ProductCategory: Pre-maderecombinantAAV;AAV-CMV-FLPo;AAV-FLPo;AAV6-CMV-FLPo;AAV6-FLPo

Description: FLPoriginallyidentifiedfromSaccharomycescerevisiaeisasite-specificrecombinases(SSR)fromtheφintegrasefamilyandrecognizesdistinct34bpFRTsequence.Ithasbeenwidelyusedforgeneratingconditionalknockoutmice,mediatedbyFLP/FRTsystem.InitialuseofFLPinmammaliancellsrevealedinefficientrecombinaseactivityduetoThermo-instABIlityoftheFLPprotein.Subsequentscreeningforthermo-stablemutantsresultedintheidentificationofFLPewhichwas4-foldincreaseinrecombinationefficiencyat37°C.HowevertherecombinationefficiencyofFLPeincellsremainsverylowbecauseofitsnon-mammalianorigin.  

FLPoisamousecodonoptimizedFLPethatgreatlyincreasestheFRTrecombinationefficiencyforbothinvivoandinvitroinmouse. ThisAAV6-CMV-FLPopre-packagedrAAVinSEROtype6(withcapsidfromAAVserotype6and2xITRfromAAVserotype2)will over-expressFLPorecombinaseunderCMVpromoter.  Readytouseformat.

Titer: >1x10^13 VG/mL

Size: > 30 µL(StdPack)

StorageBuffer: SterilePBS

InfectionProtocol: 

VectorMap: