Description:
MouseOsteocalcinFeatures&Benefits:
MouseOsteocalcinELISA
ForResearchUseOnly. Notforuseindiagnosticprocedures
Mouseosteocalcin,a46aminoacidpeptide,isthemajornoncollagenproteinfoundinmousebone.Itcontainsthreegamma-carboxyglutamicacid(GLA)residuesatpositions13,17,and20andis,therefore,alsoknownasbone
gla-proteinorBGP.TheexactBIOLOGicalfunctionofosteocalcinisnotknownbutthethreegamma-carboxyglutamicacidresiduesconferonitaverystrongABIlitytobindtohydroxyapatiteandcalcium.
TheMouseOsteocalcinELISAKitisatwo-siteenzyme-linkedimmunosorbentassay(ELISA)forthemeasurementofosteocalcininmouseserum,plasmaorcellculturemedia.Twodifferentgoatpolyclonalantibodiestomouseosteocalcinhavebeenpurifiedbyaffinitychromatography.Theantibodywhichrecognizesepitopeswithinthemidregion/C-terminalportionofthepeptideisbiotinylatedforcapture.TheotherantibodywhichrecognizesepitopeswithintheN-terminalregionisconjugatedwiththeenzymehorserADIshperoxidase(HRP)fordetection.
Feature | Benefit |
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ProductSpecifications:
ProductSpecName | ProductSpecData |
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Format | ELISA |
Tests/kit | 96wells/plate |
Sampletype | Mouseserum,plasma,cellculturemedia |
LOD | 0.4ng/mL |
Incubationtime | <3hours |