Description:
AssayKitFeatures&Benefits:
TheLyraParainfluenzaVirusAssayisamultiplexreal-timePCRassayforthequalitativedetectionandidentificationofviralnucleicacidsextractedfromnasalswabsandnasopharyngealswabsfromsymptomaticpatients.Theassayhasbeenshowntodetectanddifferentiateparainfluenzavirustypes1,2and3.
Duringthetest,amultiplexRT-PCRreactioniscarriedoutunderoptimizedconditionsinasingletubegeneratingampliconsforeachofthetargetvirusespresentinthesample.IdentificationofPIV-1,PIV-2,PIV-3andthePRCoccursbytheuseoftarget-specificprimersandfluorescent-labeledprobesthathybridizetoconservedregionsinthegenomesofPIV-1,PIV-2,PIV-3andthePRC.
TheLyraParainfluenzaVirusAssayhasaconvenient,one-stepreagentsetupperformedatroomtemperatureonthebenchtop,withouttheneedforiceorothercoolingaccessories. Moreover,optimizedcyclingconditionsmakeresultsavailableinlessthan75minutesfollowingextractiontoaidintimelydiagnosisandtreatment.LyraParainfluenzaVirusRT-PCRisavailableforuseontheAppliedBiosystems®7500FastDxReal-TimePCRInstrument.
TheLyraParainfluenzaVirusAssayprovides theflexibilityto customizerespiratorypanelswithotherLyraReal-TimePCRrespiratoryassays(LyraInfluenzaA+B,LyrahMPV+RSV,LyraAdenovirus)thatarerununderthesamecyclingconditionsinordertoadapttothe shiftingdemandscausedby variablelocalorseasonaldiseaseprevalence.
| Feature | Benefit |
|---|---|
One-stepreagentset-up | RehydrationsolutionissimplyaddedtothelyophilizedMasterMix |
Speed | Resultsinlessthan75minutesafterextraction |
Easytraining | Simplifiedanduniformworkflowwithstandardpipettingvolumes |
Refrigeratedstorage | Nofreezerneeded.2°Cto8°Cstorage |
Longshelflife | 2yearsfromdateofmanufacture |
Roomtemperaturesetup | Noiceorcoolingblockrequired |
FlexIBLe96-testformat | Kitincludesvialsof12x8MasterMix,RehydrationSolutionandliquidProcessControls |
Multi-wellmultiplexpanels | StandardizedThermocyclingconditionsfortherespiratoryassaysallowformultipleanalytestobeanalyzedonthesameplatefromthesameextractedspecimen,effectivelyprovidingacustomizablepanelofresults |
ProductSpecifications:
| ProductSpecName | ProductSpecData |
|---|---|
Sampletype | Nasalswab,Nasopharyngealswab |
Timetoresults | Lessthan75minutesafterextraction |
Reagentstorageconditions | 2°Cto8°C |
Controlsstorageconditions | 2°Cto8°C |
Sensitivity* | PIV-1:100%;PIV-2:100%;PIV-3:100% |
Specificity* | PIV-1:99.8%;PIV-2:100%;PIV-3:99.6% |
PositivePercentAgreement* | PIV-1:100%;PIV-2:100%;PIV-3:100% |
NegativePercentAgreement* | PIV-1:98.8%;PIV-2:94.0%;PIV-3:100% |
LOD | PIV-1:2.50x100;PIV-2:2.50x102;PIV-3:8.00x101(TCID50/mL) |
Samplevolumeneededforextraction | 180µL |
Purifiednucleicacidvolumeneededtotesting | 5µL |
Shelflife | 24months |
*RefertoPackageInsertforadditionalperformanceclaims.
