- Protein Expression|Protein Purification|Protein Tools
- DNA Modifying Enzymes and Cloning Technologies|Next Generation Sequencing Library Preparat
- DNA修饰酶
- DNA Assembly Cloning and Mutagenesis Kits
- DNA修饰酶和克隆技术|NGS样品制备&;目标富集|PCR
- 缓冲液|下一代测序文库制备|NGS样品制备&;目标富集
- Protein Expression|Protein Expression & Purification Technologies|Protein Purification
- 表观遗传学|限制性内切酶
- DNA Modifying Enzymes and Cloning Technologies|Epigenetics
- Nucleic Acid Purification|RNA Reagents
- 表观遗传学
- DNA Modifying Enzymes and Cloning Technologies
- Buffers|DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplificatio
- Buffers|Markers & Ladders|RNA Reagents
- Next Generation Sequencing Library Preparation|NGS Sample Prep & Target Enrichment
- Nucleic Acid Purification|Protein Expression & Purification Technologies|RNA Reagents
- Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno
- Buffers|DNA Modifying Enzymes and Cloning Technologies
- Buffers|Competent Cells
- DNA Modifying Enzymes and Cloning Technologies|Next Generation Sequencing Library Preparat
- Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno
Description:
ThePh.D.-7PhageDisplayPeptideLibraryisbasedonacombinatoriallibraryofrandomheptapeptidesfusedtoaminorcoatprotein(pIII)ofM13phage(1–6).Thedisplayedpeptide(7-mer)isexpressedattheN-terminusofpIII,i.e.,thefirstresidueofthematureproteinisthefirstrandomizedposition.Thepeptideisfollowedbyashortspacer(Gly-Gly-Gly-Ser)andthenthewild-typepIIIsequence.Thelibraryconsistsofapproximately109electroporatedsequencesamplifiedoncetoyieldapproximately100copiesofeachsequencein10µlofthesuppliedphage.
CitationsforPhageDisplayPanningwithapentavalentpeptidelibrarydisplayedonplll.
KitComponents
Thefollowingreagentsaresuppliedwiththisproduct:
Storeat(°C) | Concentration | |
E.coliK12ER2738 | -70 | |
-28glllSequencingPrimer(22-mer) | -20 | 1pmol/μl |
-96glllSequencingPrimer(20-mer) | -20 | 1pmol/μl |
Ph.D.™-7PhageDisplayPeptideLibrary | -20 | 1x1013pfu/ml |
Streptavidin,lyophilized | -20 | |
Biotin | -20 | 10mM |