品牌分类
- Markers & Ladders|Protein Tools
- PCR, Polymerases & Amplification Technologies|RNA Reagents
- DNA Modifying Enzymes and Cloning Technologies|Epigenetics
- Next Generation Sequencing Library Preparation|NGS Sample Prep & Target Enrichment|PCR
- Competent Cells|Protein Expression|Protein Expression & Purification Technologies|Prot
- Nucleic Acid Purification|PCR, Polymerases & Amplification Technologies|RNA Reagents
- Next Generation Sequencing Library Preparation|NGS Sample Prep & Target Enrichment|PCR
- DNA Modifying Enzymes and Cloning Technologies|Nucleic Acid Purification
- Competent Cells|Protein Expression|Protein Expression & Purification Technologies|Prot
- PCR, Polymerases & Amplification Technologies|Protein Expression|Protein Expression &a
- 标记&;梯子
- Glycobiology|Protein Tools
- Nucleic Acid Purification|Protein Expression & Purification Technologies|RNA Reagents
- Next Generation Sequencing Library Preparation|PCR, Polymerases & Amplification Techno
- DNA修饰酶
- DNA Modifying Enzymes and Cloning Technologies|PCR, Polymerases & Amplification Techno
- Competent Cells|Protein Expression & Purification Technologies
- Buffers|Nucleic Acid Purification
- 感受态细胞|蛋白质表达|蛋白质表达&;净化技术|Prot
- Cellular Analysis|Protein Tools
- DNA Assembly Cloning and Mutagenesis Kits|DNA Modifying Enzymes and Cloning Technologies
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
WarmStart RTx Reverse Transcriptase is a unique in silico designed RNA-directed DNA polymerase coupled with a reversibly-bound aptamer that inhibits RTx activity below 40°C. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA (cDNA synthesis) or single-stranded DNA as a template. RTx is a robust enzyme for RNA detection in amplification reactions and is particularly well suited for use in LAMP (loop-mediated isothermal amplification). The WarmStart property enables high throughput applications, room temperature setup, and increases the consistency and specificity of amplification reactions. RTx contains intact RNase H activity.
Sensitive Detection with Reduced False Negatives
RT-LAMP reactions were performed with WarmStart RTx and AMV Reverse Transcriptase with total RNA at 6 input concentrations. Positive detection, indicated by fluorescent detection with a twenty-minute reaction time, was achieved down to 1 pg of total input RNA. Use of AMV resulted in false negatives when total RNA was 100 pg or lower.
| Total RNA | WarmStart RTx | AMV RT |
| 100 ng | 3/3 | 3/3 |
| 10 ng | 3/3 | 3/3 |
| 1 ng | 3/3 | 3/3 |
| 100 pg | 3/3 | 2/3 |
| 10 pg | 3/3 | 2/3 |
| 1pg | 3/3 | 1/3 |


- This product is related to the following categories:
- RT-PCR Products,
- Reverse Transcriptases & RT-PCR Products,
- cDNA Synthesis & Reverse Transcriptases Products
- This product can be used in the following applications:
- Strand Displacement Amplification & Nicking Enzyme,
- cDNA Synthesis,
- Reverse Transcription (cDNA Synthesis),
- Loop-Mediated Isothermal Amplification,
- RT-PCR & cDNA Synthesis,
- RT-qPCR, RT-PCR and cDNA Synthesis,
- Isothermal Amplification, PCR


