Immunogen	Polyclonal LC3B Antibody was made to a synthetic peptide made to the N-terminal region of the human LC3B protein. [Uniprot: Q9GZQ8]
Localization	LC3-I is cytoplasmic. LC3-II binds to the autophagic membranes.
Marker	Autophagosome Marker
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	MAP1LC3B
Purity	Immunogen affinity purified
Innovator"s Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator"s Reward

Dilutions	Western Blot 1:1000 Simple Western 1:100 Electron Microscopy ELISA Flow Cytometry 1:200 Immunoblotting Immunocytochemistry/Immunofluorescence 0.1-2 ug/ml Immunohistochemistry Immunohistochemistry-Frozen 1:400 Immunohistochemistry-Paraffin 1:200-1:400 Immunoprecipitation Immunohistochemistry Free-Floating Knockdown Validated Knockout Validated
Application Notes	In Western Blot, bands are seen at ~17 and 19 kDa corresponding to LC3-II and LC3-I. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Electron Microscopy was reported in scientific literature (PMID: 21885071). Use in Immunohistochemistry-free floating reported in scientific literature (PMID: 24928515). Use in Immunohistochemistry on both paraffin-embedded and frozen sections reported in scientific literature (PMID: 18259115). Use in immunoprecipitation reported in scientific literature (PMID: 26098573). Use in immunoblotting reported in scientific literature (PMID: 25383539). Use in flow reported in scientific literature (PMID: 27622036). Use in ELISA reported in scientific literature (PMID: 21885071). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW	14.688 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control	Human Brain Hippocampus Membrane Tissue Lysate (Adult Membrane Normal) Human Brain Whole Tissue Lysate (Adult Whole Normal) Neuro2a Whole Cell Lysate Neuro2a Chloroquine Treated / Untreated Cell Lysate HeLa Chloroquine Treated / Untreated Cell Lysate
Reviewed Applications	Read 18 Reviews rated 4.2 using NB600-1384 in the following applications: Western Blot Immunofluorescence Immunocytochemistry Immunohistochemistry
Publications	ReadPublications using NB600-1384 in the following applications: EM 2 publications FLOW 4 publications Flow 4 publications IB 1 publication ICC 1 publication ICC/IF 50 publications IHC 14 publications IHC-Fr 2 publications IHC-FrFl 1 publication IHC-P 7 publications IP 1 publication KD 1 publication WB 195 publications

Novus Biologicals北京代理，Novus Biologicals中国代理，Novus Biologicals上海代理，Novus Biologicals公司，Novus Biologicals 【详细说明】  Novus Biologicals北京代理，Novus Biologicals中国代理，Novus Biologicals上海代理，Novus Biologicals公司，Novus Biologicals 上海普迈生物科技有限公司签约代理      欢迎下载下面的资料以了解此产品的相关信息！预询产品订购信息  上海普迈生物致力于提供zui高质量的产品和服务组织形态学研究与竞争力的产品 产品和服务,有相关产品的问题请直接 期货每周下午5点前发定单，正常到上海时间为3-4周 现市区2天内送到，外地以快递为准 敬请关注！ ：   :www.pumaibio.com :pumaibio :（直接客户） （经销商） 促销etonbio、polysciences、Mattek、trevigen、Chromogenix、cyguns、IrvineScientific、Innovative Cell Technologies、enzo、novus、immunochemistry、eylabs、Polyplus、NIBSC、ALZET、hitobiotec、sutter instrument、mirus、StressMarq、Avanti、jena bioscience、Neuro Probe、tedpella、avanti、Rockland、CAYMAN、transgenomic、BioLegend、ambion、omicronbio、 Assaybiotech、chondrex、lc?laboratories、cosmobio、megazyme、NeoMarkers、BTI、eylabs、berryassoc、cellagentech、TOCRIS、Relia Tech、SouthernBiotech、Biosearch technology、Lumiprobe、StemCells?Inc、biovendor、NORGEN、alomone、jena?bioscience、Gracebio、Eurogentec、Gelcompany?、invitrogen、gene?bridges、MRC-Holland、laysanbio