Immunogen	This LC3A Antibody was prepared from a synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121). [Uniprot: Q9H492].
Localization	LC3-I is cytoplasmic. LC3-II binds to the autophagic membranes.
Marker	Autophagosome Marker
Specificity	This LC3A Antibody detects both LC3A and LC3B.
Predicted Species	Bovine (100%). Backed by our 100% Guarantee.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	MAP1LC3A
Purity	Immunogen affinity purified
Innovator"s Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator"s Reward

Dilutions	Western Blot Simple Western 1:50 Chromatin Immunoprecipitation ELISA Flow Cytometry Immunoblotting Immunocytochemistry/Immunofluorescence 1:100-1:300 Immunohistochemistry 1:200-1:400 Immunohistochemistry-Frozen Immunohistochemistry-Paraffin 1:200-1:400 Immunoprecipitation 20 ug / 500 ug of lysate Southern Blot Immunohistochemistry Whole-Mount
Application Notes	Western blot bands are seen at ~19 kDa, representing LC3-I, and ~17 kDa, representing LC3-II. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications,post translation cleavages, relative charges, and other experimental factors.In ICC, cytoplasmic staining was observed in HeLa cells.Use in immunocytochemistry (PMID: 21545732). and Immunohistochemistry-Paraffin (PMID: 26571030). reported in scientific literature. Use in Flow cytometry reported in scientific literature ( PMID: 24419333).. Use in ELISA reported in scientific literature (PMID: 20930550).. Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID: 31783118)..Use in Southern blot reported in scientific literature (PMID: 21262964).. Use in immunoblotting reported in scientific literature (PMID: 28253371).. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Use in Chromatin Immunoprecipitation reported in scientific literature (PMID:33035707).
Control	LC3B Overexpression Lysate Mouse Brain Whole Tissue Lysate (Adult Whole Normal) Human Brain Whole Tissue Lysate (Adult Whole Normal) Neuro2a Whole Cell Lysate Neuro2a Chloroquine Treated / Untreated Cell Lysate HeLa Chloroquine Treated / Untreated Cell Lysate
Reviewed Applications	Read 20 Reviews rated 4.4 using NB100-2331 in the following applications: Western Blot Immunofluorescence Immunohistochemistry-Paraffin Immunohistochemistry
Publications	ReadPublications using NB100-2331 in the following applications: ChIP 1 publication ELISA 2 publications FLOW 1 publication IB 1 publication ICC/IF 35 publications IHC 9 publications IHC-Fr 6 publications IHC-P 4 publications IHC-WhMt 1 publication IP 3 publications WB 153 publications WB,IP 1 publication

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