Immunogen	This HIF-1 alpha antibody was developed against a fusion protein including amino acids 530 - 825 of the mouse HIF-1 alpha protein [Uniprot# Q61221].
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	HIF1A
Purity	Immunogen affinity purified
Innovator"s Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator"s Reward

Dilutions	Western Blot Chromatin Immunoprecipitation 1:10 - 1:500 Flow Cytometry 1:10 - 1:1000 Immunoblotting Immunocytochemistry/Immunofluorescence 1:100 Immunohistochemistry 5 - 10 ug/mL Immunohistochemistry-Frozen 5 - 10 ug/mL Immunohistochemistry-Paraffin 5 - 10 ug/mL Immunoprecipitation 1:10 - 1:500 Immunohistochemistry Whole-Mount
Application Notes	WB recognizes a band in hypoxic samples at ~115 kDa. HIF-1 alpha protein (NBC1-18422) has been successfully used for antibody preadsorption/blocking assay in IHC-P with HIF-1 alpha antibody NB100-479. Flow Cytometry usage reported in scientific literature (PMID 21917971). Use in Immunoblotting reported in scientific literature (PMID 28506759). Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID: 27148974).
Theoretical MW	93 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control	Human Pancreas Whole Tissue Lysate (Adult Whole Normal) COS-7 Nuclear Hypoxic Induced Cell Lysate Human Bone Marrow Whole Tissue Lysate (Adult Whole Diabetes) HeLa CoCl2 treated/Untreated Cell Lysate HepG2 CoCl2 treated/Untreated Cell Lysate HeLa Hypoxic / Normoxic Cell Lysate HepG2 Hypoxic / Normoxic Cell Lysate HIF-1 alpha Knockout HeLa Cell Lysate
Control Peptide	HIF-1 alpha Recombinant Protein (NBC1-18422)
Reviewed Applications	Read 24 Reviews rated 4.4 using NB100-479 in the following applications: Western Blot Chromatin Immunoprecipitation Immunohistochemistry-Paraffin
Publications	ReadPublications using NB100-479 in the following applications: ChIP 8 publications FLOW 2 publications IB 1 publication ICC/IF 32 publications IHC 37 publications IHC-Fr 6 publications IHC-P 34 publications IHC-WhMt 1 publication IP 6 publications WB 225 publications WB,ChIP 1 publication

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