Immunogen	Partial peptide sequence (within residues 1100-1300) of human ABCA1 Antibody [UniProt# O95477].Actual immunogen sequence is proprietary information.
Localization	Astrocyte Marker
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	ABCA1
Purity	Immunogen affinity purified
Innovator"s Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator"s Reward

Dilutions	Western Blot 1:500 Chromatin Immunoprecipitation ELISA Flow Cytometry 1:400 Immunoblotting Immunocytochemistry/Immunofluorescence 1:100 Immunohistochemistry 1:200 Immunohistochemistry-Frozen Immunohistochemistry-Paraffin 1:200 Immunoprecipitation 1:10-1:500 Block/Neutralize Dual RNAscope ISH-IHC Gel Supershift Assay Knockdown Validated Knockout Validated PCR
Application Notes	This ABCA1 antibody has been used for Flow Cytometry and ICC (PMID: 21501868), IP (PMID: 21846716; 21106522). Use in Gel supper shift assay reported in scientific literature (PMID 15684432). Use in immunoblotting reported in scientific literature (PMID: 27599291). Use in blocking/ neutralizing reported in scientific literature (PMID: 30821416). Use in PCR reported in scientific literature (PMID: 27406916). Western Blot band representing ABCA1 is observed at approx. 220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal. Chromatin Immunoprecipitation (PMID 19515742) and ELISA (PMID 18541924) were also reported in scientific literature. Knockdown validation (PMID: 31666189). Knockout validation (PMID: 32273567). In Simple Western only 10-15 uL of the recommended dilution is used per data point. Separated by Charge.
Theoretical MW	220 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control	Raw 264.7 Whole Cell - T0901317 treated Assay Kit
Control Peptide	ABCA1 Protein (NB400-105PEP)
Reviewed Applications	Read 6 Reviews rated 4.3 using NB400-105 in the following applications: Western Blot Immunofluorescence
Publications	ReadPublications using NB400-105 in the following applications: B/N 1 publication ChIP 1 publication ELISA 1 publication EMSA 1 publication FLOW 1 publication Flow 2 publications IB 1 publication ICC/IF 20 publications IHC 16 publications IHC-FR 1 publication IHC-Fr 3 publications IHC-P 7 publications IP 9 publications KD 1 publication KO 1 publication WB 188 publications

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