Immunogen	This Survivin Antibody was developed against full length recombinant human Survivin [UniProt# O15392]
Localization	Nuclear
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	BIRC5
Purity	Immunogen affinity purified
Innovator"s Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator"s Reward

Dilutions	Western Blot 1:1000 Simple Western 1:25 Chromatin Immunoprecipitation 1:10-1:500 ELISA Flow Cytometry Immunocytochemistry/Immunofluorescence 1:50-1:250 Immunohistochemistry 1:50-1:100 Immunohistochemistry-Frozen Immunohistochemistry-Paraffin 1:50-1:500 Immunoprecipitation 1:10-1:500 Dual RNAscope ISH-IHC Knockdown Validated
Application Notes	In WB, a band at approx. 16.5 kDa can be seen. For IHC, prior antigen retrieval (pressure cooking) is recommended for cytoplasmic and nuclear detection of Survivin. Immunohistochemistry-Frozen, Flow Cytometry, and ELISA were reported in scientific literature (PMIDs: 12671708, 17875988, 24102797). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW	16 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control	HeLa Whole Cell Lysate Survivin Overexpression Lysate
Reviewed Applications	Read 21 Reviews rated 4.4 using NB500-201 in the following applications: Western Blot Immunohistochemistry-Paraffin Immunocytochemistry ELISA
Publications	ReadPublications using NB500-201 in the following applications: ELISA 2 publications FLOW 2 publications Flow 2 publications ICC/IF 29 publications IHC 42 publications IHC-Fr 2 publications IHC-P 37 publications IP 4 publications KD 3 publications PLA 2 publications WB 112 publications

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