Summary:

Immunogen	ApeptidederivedfromtheC-terminusofmouse/humanHIF-2alphaprotein.
Specificity	SpecificforHIF-2alpha/EPAS.Doesnotcross-reactwithHIF-1alpha.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	EPAS1
Purity	Immunogenaffinitypurified
Innovator"sReward	Testinaspecies/applicationnotlistedabovetoreceiveafullcredittowardsafuturepurchase. LearnabouttheInnovator"sReward

Applications/Dilutions:

Dilutions	WesternBlot1.0ug/ml-2.0ug/ml SimpleWestern1:50 ChromatinImmunoprecipitation1:10-1:500 ELISA1:100-1:2000 FlowCytometry GelSuperShiftAssays Immunoblotting Immunocytochemistry/Immunofluorescence1:100 Immunohistochemistry1:100 Immunohistochemistry-Frozen Immunohistochemistry-Paraffin1:100 Immunoprecipitation5ug/1mglysate
ApplicationNotes	ThisHIF-2alphaantibodyisusefulforWesternblot,Immunoprecipitation,ChromatinImmunoprecipitation,ELISA,GelShiftSuperAssay(PMID:15184875),UseinImmunoblottingreportedinscientificliterature(PMID28115701)..Immunocytochemistry/ImmunofluorescenceandImmunohistochemistryonparaffin-embeddedsections.InWB,thisantibodyrecognizesabandat118kDarepresentingHIF-2alpha. InSimpleWesternonly10-15uLoftherecommendeddilutionisusedperdatapoint.SeparatedbySize-Wes,SallySue/PeggySue. Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.
TheoreticalMW	118kDa. Disclaimernote:Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.
PositiveControl	PC-12Lysate(NB810-55229) COS-7Lysate(NB800-PC26) RectumLysate(NB820-59250)
ReviewedApplications	Read28Reviewsrated4.4 using NB100-122inthefollowingapplications: WesternBlot ChromatinImmunoprecipitation Immunohistochemistry-Paraffin Immunofluorescence
Publications	ReadPublicationsusing NB100-122inthefollowingapplications: ChIP 38publications Co-IP 1publication ELISA 5publications FLOW 3publications Flow 1publication GS 2publications IB 20publications ICC/IF 18publications IHC 21publications IHC-Fr 4publications IHC-P 28publications IP 16publications WB 319publications

ReactivityNotes:

Reactivitywithsheepreportedinthescientificliterature(PMID:17307811).Hamsterreactivityreportedinscientificliterature(PMID:24997364and24997360)

Packaging,Storage&Formulations:

Storage	Storeat-20°C.
Buffer	PBS
Preservative	0.05%SodiumAzide
Concentration	1.0mg/ml
Purity	Immunogenaffinitypurified

Notes:

• antihif2alpha
• anti-HIF-2alpha
• Basic-helix-loop-helix-PASproteinMOP2
• BHLHE73
• bHLHe73ECYT4
• ClassEbasichelix-loop-helixprotein73
• endothelialPASdomainprotein1
• endothelialPASdomain-containingprotein1
• EPAS1
• EPAS-1
• HIF2A
• HIF-1alpha-likefactor
• HIF2alpha
• HIF-2alpha
• hif2aangiogenesis
• HIF2A
• HIF2AHIF-2-alpha
• HLF
• HLFHIF-1-alpha-likefactor
• hypoxia-inducIBLefactor2alpha
• Hypoxia-induciblefactor2-alpha
• MemberofPASprotein2
• MOP2
• MOP2HIF2-alpha
• PASD2PASdomain-containingprotein2

AlternateNamesfor53BP1Antibody:

Hypoxia-induciblefactors(HIFs)generateheterodimerscomposedofalpha-subunits(HIF-1alpha,HIF-2alphaandHIF-3alpha)andbetasubunits(Arnt1,Arnt2andArnt3).Oftheseisoforms,HIF-1alphaandHIF-2alphaplaycriticalrolesinthecellularresponsetohypoxicconditions.AfterheterodimerizationwithbetasubunitsbindingtoDNAsequence5"-[AG]CGTG-3"withinHRE(hypoxiaresponseelement)oftargetgenepromoters,HIF-2alpharegulatesthegeneexpressioninresponsetooxygenhomeostasisalterations.HIF-2alpharegulatesVEGFexpressioninvolvedinthedevelopmentofnewbloodvesselsaswellasthetubularsystemofthelung.HIF-2alphaplaysaroleintheformationoftheendotheliumthatgivesrisetobloodbrainbarrier.HIF-2alphaisastrongactivatorofexpressionofTie-2tyrosinekinase,whichisamajorplayerinangiogenesis.HIF-2alphaactivationrequiresrecruitmentoftranscriptionalcoactivatorssuchasCREBPB,EP300,anditsinteractionwithredoxregulatoryproteinAPEXmediatesactivationofCTAD.HIF-2alphaandHIF-1alphaexpressionincreasesincertainmalignancies;wherein,HIF-1alphaplaysbothpro-aswellasanti-proliferativeactivities.HIF-2alphaissuggestedtoexertanti-proliferativeactivityonly.

Background:

Thisproductisforresearchuseonlyandisnotapprovedforuseinhumansorinclinicaldiagnosis.PrimaryAntibodiesareguaranteedfor1yearfromdateofreceipt.

Limitations:

Hypoxia-induciblefactors(HIFs)generateheterodimerscomposedofalpha-subunits(HIF-1alpha,HIF-2alphaandHIF-3alpha)andbetasubunits(Arnt1,Arnt2andArnt3).Oftheseisoforms,HIF-1alphaandHIF-2alphaplaycriticalrolesinthecellularresponsetohypoxicconditions.AfterheterodimerizationwithbetasubunitsbindingtoDNAsequence5"-[AG]CGTG-3"withinHRE(hypoxiaresponseelement)oftargetgenepromoters,HIF-2alpharegulatesthegeneexpressioninresponsetooxygenhomeostasisalterations.HIF-2alpharegulatesVEGFexpressioninvolvedinthedevelopmentofnewbloodvesselsaswellasthetubularsystemofthelung.HIF-2alphaplaysaroleintheformationoftheendotheliumthatgivesrisetobloodbrainbarrier.HIF-2alphaisastrongactivatorofexpressionofTie-2tyrosinekinase,whichisamajorplayerinangiogenesis.HIF-2alphaactivationrequiresrecruitmentoftranscriptionalcoactivatorssuchasCREBPB,EP300,anditsinteractionwithredoxregulatoryproteinAPEXmediatesactivationofCTAD.HIF-2alphaandHIF-1alphaexpressionincreasesincertainmalignancies;wherein,HIF-1alphaplaysbothpro-aswellasanti-proliferativeactivities.HIF-2alphaissuggestedtoexertanti-proliferativeactivityonly. Novus Biologicals北京代理，Novus Biologicals中国代理，Novus Biologicals上海代理，Novus Biologicals公司，Novus Biologicals 【详细说明】  Novus Biologicals北京代理，Novus Biologicals中国代理，Novus Biologicals上海代理，Novus Biologicals公司，Novus Biologicals 上海普迈生物科技有限公司签约代理      欢迎下载下面的资料以了解此产品的相关信息！预询产品订购信息  上海普迈生物致力于提供zui高质量的产品和服务组织形态学研究与竞争力的产品 产品和服务,有相关产品的问题请直接 期货每周下午5点前发定单，正常到上海时间为3-4周 现市区2天内送到，外地以快递为准 敬请关注！ ：   :www.pumaibio.com :pumaibio :（直接客户） （经销商） 促销etonbio、polysciences、Mattek、trevigen、Chromogenix、cyguns、IrvineScientific、Innovative Cell Technologies、enzo、novus、immunochemistry、eylabs、Polyplus、NIBSC、ALZET、hitobiotec、sutter instrument、mirus、StressMarq、Avanti、jena bioscience、Neuro Probe、tedpella、avanti、Rockland、CAYMAN、transgenomic、BioLegend、ambion、omicronbio、 Assaybiotech、chondrex、lc?laboratories、cosmobio、megazyme、NeoMarkers、BTI、eylabs、berryassoc、cellagentech、TOCRIS、Relia Tech、SouthernBiotech、Biosearch technology、Lumiprobe、StemCells?Inc、biovendor、NORGEN、alomone、jena?bioscience、Gracebio、Eurogentec、Gelcompany?、invitrogen、gene?bridges、MRC-Holland、laysanbio