Summary:

Immunogen	AsyntheticpeptidemadetoanN-terminalportionofthehumanLC3proteinsequence(betweenresidues1-100).[UniProt#Q9GZQ8]
Localization	LC3-Iiscytoplasmic.LC3-IIbindstotheautophagicmembranes.
Marker	AutophagosomeMarker
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	MAP1LC3B
Purity	Immunogenaffinitypurified
Innovator"sReward	Testinaspecies/applicationnotlistedabovetoreceiveafullcredittowardsafuturepurchase. LearnabouttheInnovator"sReward

Applications/Dilutions:

Dilutions	WesternBlot0.5-2ug/ml SimpleWestern1:50 ELISA Immunocytochemistry/Immunofluorescence1:200 Immunohistochemistry1:200-1:400 Immunohistochemistry-Frozen Immunohistochemistry-Paraffin1:200-1:400 Immunoprecipitation20ug/500ugofprotein ProximityLigationAssay KnockoutValidated
ApplicationNotes	ThisLC3antibodyisusefulforImmunocytochemistry/Immunofluorescence,Immunohistochemistry-paraffinembeddedsections,ImmunoprecipitationandWesternBlot.UseinImmunohistochemistry-Frozensectionsreportedinscientificliterature(PMID:20008275).UseinELISAreportedinscientificliterature(PMID20503249).InWesternblotbandsareseen~17and19kDacorrespondingtoLC3-IIandLC3-I.Insomecasesanon-specificbandisseenat~21kDainmouseprotein.InICC/IF,cytoplasmicstainingwasobservedinHeLacells.UseinProximityLigationAssayreportedinscientificliterature(PMID27219062). InSimpleWesternonly10-15uLoftherecommendeddilutionisusedperdatapoint.SeparatedbySize-Wes,SallySue/PeggySue.
PositiveControl	LC3BLysate(NBL1-12844) BrainCerebralCortexLysate(NB820-59182) BrainLysate(NB820-59177) Neuro2aLysate(NBP2-19017) Neuro2aLysate(NBP2-49688) HeLaLysate(NBP2-49689)
ControlPeptide	LC3BPeptide(NB100-2220PEP)
ReviewedApplications	Read33Reviewsrated4.7 using NB100-2220inthefollowingapplications: Immunohistochemistry-Frozen WesternBlot Immunofluorescence SimpleWestern Immunohistochemistry Immunohistochemistry-Paraffin Immunoprecipitation
Publications	ReadPublicationsusing NB100-2220inthefollowingapplications: ChIP 1publication ELISA 2publications FLOW 3publications IB 4publications ICC/IF 89publications IHC 25publications IHC-Fr 7publications IHC-P 14publications IP 2publications PLA 1publication WB 412publications

ReactivityNotes:

Bovinereactivityreportedinscientificliterature(PMID:24895572).Primatereactivityreportedinscientificliterature(PMID:25142602).Caninereactivityreportedinscientificliterature(PMID:25839646).Themousedetectionhasbeenreportedtobeweakerthanthehuman.Immunogensequencehas84%homologytoXenopus.

Packaging,Storage&Formulations:

Storage	Aliquotandstoreat-20Cor-80C.Avoidfreeze-thawcycles.
Buffer	PBS
Preservative	0.02%SodiumAzide
Concentration	1.0mg/ml
Purity	Immunogenaffinitypurified

Notes:

• anti-LC3B
• Apg8b
• atg8fantibody
• ATG8F
• Autophagy-relatedproteinLC3B
• Autophagy-relatedubiquitin-likemodifierLC3B
• lc3bautophagymarker
• LC3Bihc
• LC3Bimmunohistochemistry
• LC3Bimmunoprecipitation
• LC3Bwesternblot
• LC3B
• lc3-i,ii
• LC3II
• MAP1lightchain3-likeprotein2
• MAP1A/1BLC3
• MAP1A/MAP1BLC3B
• MAP1LC3B
• microtubuleassociatedprotein3b
• microtubuleassociatedprotein3beta
• microtubule-associatedprotein1lightchain3betaMAP1A/MAP1Blightchain3B
• microtubule-associatedproteins1A/1Blightchain3
• microtubule-associatedproteins1A/1Blightchain3B

AlternateNamesfor53BP1Antibody:

LC3(microtubule-associatedproteinlightchain3),themoststudiedautophagybiomarker,wasoriginallyidentifiedasasubunitofmicrotubule-associatedproteins1Aand1B(MAP1LC3)andwaslaterfoundtocontainsimilaritytoyeastproteinApg8/Aut7/Cvt5.Distributedubiquitouslyineukaryotes,LC3isexpressedas3splicevariants/isoforms(LC3A,LC3BandLC3C)whichundergopost-translationalprocessing,wherein,theunprocessedformofLC3isproteolyticallycleavedbyAtg4proteasetoformLC3-Iwithcarboxyterminalexposedglycine.Duringautophagy,thisexposedglycineofLC3-IisconjugatedbyAtg7(anE1-likeactivity),Atg3(anE2-likeconjugatingactivity)andbyAtg12-Atg5-Atg16Lmultimers(E3-likeligaseactivity)tophosphatidylethanolamine(PE)moietyforgeneratingLC3-II.ThelipophiliccharacterofPEgroupfacilitatesLC3-IIinsertionintoautophagosomesmembranes,andasaresultLC3-IIisdegradedwhenautophagosomesfusewithlysosomestoformautolysosomesforlysusofintra-autophagosomalcomponentsbylysosomalhydrolases.ConversionofLC3ItoLC3IIwhencorrelatedwithautophagosomenumbersisconsideredasthebestmarkerofautophagybecauseLC3-IIistheonlywell-characterizedproteinwhichspecificallylocalizetoautophagicstructuresthroughoutautophagy(fromphagophoretolysosomaldegradation).LC3isagreattoolinresearchasautophagyisimplicatedinnumerousphysiological/pathologicalprocessesincludingresponsestoexercise/aging,cancer,metabolicandneurodegenerativedisorders,andcardiovascular/pulmonarydiseases.

Background:

Thisproductisforresearchuseonlyandisnotapprovedforuseinhumansorinclinicaldiagnosis.PrimaryAntibodiesareguaranteedfor1yearfromdateofreceipt.

Limitations:

LC3(microtubule-associatedproteinlightchain3),themoststudiedautophagybiomarker,wasoriginallyidentifiedasasubunitofmicrotubule-associatedproteins1Aand1B(MAP1LC3)andwaslaterfoundtocontainsimilaritytoyeastproteinApg8/Aut7/Cvt5.Distributedubiquitouslyineukaryotes,LC3isexpressedas3splicevariants/isoforms(LC3A,LC3BandLC3C)whichundergopost-translationalprocessing,wherein,theunprocessedformofLC3isproteolyticallycleavedbyAtg4proteasetoformLC3-Iwithcarboxyterminalexposedglycine.Duringautophagy,thisexposedglycineofLC3-IisconjugatedbyAtg7(anE1-likeactivity),Atg3(anE2-likeconjugatingactivity)andbyAtg12-Atg5-Atg16Lmultimers(E3-likeligaseactivity)tophosphatidylethanolamine(PE)moietyforgeneratingLC3-II.ThelipophiliccharacterofPEgroupfacilitatesLC3-IIinsertionintoautophagosomesmembranes,andasaresultLC3-IIisdegradedwhenautophagosomesfusewithlysosomestoformautolysosomesforlysusofintra-autophagosomalcomponentsbylysosomalhydrolases.ConversionofLC3ItoLC3IIwhencorrelatedwithautophagosomenumbersisconsideredasthebestmarkerofautophagybecauseLC3-IIistheonlywell-characterizedproteinwhichspecificallylocalizetoautophagicstructuresthroughoutautophagy(fromphagophoretolysosomaldegradation).LC3isagreattoolinresearchasautophagyisimplicatedinnumerousphysiological/pathologicalprocessesincludingresponsestoexercise/aging,cancer,metabolicandneurodegenerativedisorders,andcardiovascular/pulmonarydiseases. Novus Biologicals北京代理，Novus Biologicals中国代理，Novus Biologicals上海代理，Novus Biologicals公司，Novus Biologicals 【详细说明】  Novus Biologicals北京代理，Novus Biologicals中国代理，Novus Biologicals上海代理，Novus Biologicals公司，Novus Biologicals 上海普迈生物科技有限公司签约代理      欢迎下载下面的资料以了解此产品的相关信息！预询产品订购信息  上海普迈生物致力于提供zui高质量的产品和服务组织形态学研究与竞争力的产品 产品和服务,有相关产品的问题请直接 期货每周下午5点前发定单，正常到上海时间为3-4周 现市区2天内送到，外地以快递为准 敬请关注！ ：   :www.pumaibio.com :pumaibio :（直接客户） （经销商） 促销etonbio、polysciences、Mattek、trevigen、Chromogenix、cyguns、IrvineScientific、Innovative Cell Technologies、enzo、novus、immunochemistry、eylabs、Polyplus、NIBSC、ALZET、hitobiotec、sutter instrument、mirus、StressMarq、Avanti、jena bioscience、Neuro Probe、tedpella、avanti、Rockland、CAYMAN、transgenomic、BioLegend、ambion、omicronbio、 Assaybiotech、chondrex、lc?laboratories、cosmobio、megazyme、NeoMarkers、BTI、eylabs、berryassoc、cellagentech、TOCRIS、Relia Tech、SouthernBiotech、Biosearch technology、Lumiprobe、StemCells?Inc、biovendor、NORGEN、alomone、jena?bioscience、Gracebio、Eurogentec、Gelcompany?、invitrogen、gene?bridges、MRC-Holland、laysanbio