Description	NBP2-49689 contains 1 vial each of 0.1ml of HeLa Chloroquine treated and 0.1ml of HeLa untreated cell lysate. This lysate set is useful as a positive and negative controls for targets such as LC3, p62 etc. and the researchers should use anti-Human primary antibodies when working with these lysates.
Marker	Autophagy/LC3 Positive Control
PreparationMethod	HeLa cells were cultured under standard laboratory conditions until semi-confluent (70-80%).The cells were then treated with or without Chloroquine to 50 uM for 24 hours.The cells were washed in PBS and directly lysed into 1X Laemmli sample buffer containing BME.Each lysate is sonicated and boiled before being tested in Western blot for reactivity to LC3.Tubulin reactivity in each lysate is shown as a loading control.

Application Notes

HeLa Chloroquine treated / untreated whole cell lysates are provided as positive and negative control for Western blot analysis in Autophagy research. The lysates are provided as one vial of treated and one vial of untreated samples.Use 10 ul per lane for a standard mini-gel blot (approx. 1 mg/ml).

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